正交设计优化南苍术快速繁殖培养基的研究

目的]保护野生苍术资源,并为南苍术工厂化生产提供技术指导。方法]以南苍术Atractylodes lancea(Thunb.)DC]种子为材料得到无菌试管苗,应用正交试验法研究6-苄基腺嘌呤(6-BA)、萘乙酸(NAA)、激动素(KT)及其配比对南苍术无菌苗增殖的影响;同时研究了NAA浓度对南苍术苗生根的影响;以泥土、河沙、棉籽壳为材料,采用正交试验法设计9种轻型基质配方,研究其对南苍术生长的影响。结果]适合南苍术试管苗增殖的培养基为MS+6-BA 1.0 mg/L+NAA 0.4 mg/L+KT 0.4 mg/L;适合南苍术生根的培养基为1/2MS+NAA 0.5 mg/L;最适基质配比为泥土∶河沙∶棉籽壳=3∶3∶2(体积比)。结论]筛选出了南苍术增殖培养和生根的最佳配方,以及基质的最佳配比,为苍术资源的实验室保存和种苗工厂化生产提供了技术依据。

Study on Tissue Culture of Atractylodes lances by Orthogonal Design

Objective] The aim was to protect wild resource of Atractylodes lances and to provide technical guidance for industrialization of Atractylodes lances.Method] The test-tube seedlings were produced from seeds of Atractylodis lances.At the same time,the effects of 6-BA,NAA and KT on the proliferation of aseptic seedling was studied through the orthogonal test.The effect of NAA concentration on the rooting of aseptic seedling was studied through nine types of light substrate groups,which consist of peat soil,fine sand and cotton seed hull,and their effects on growth of Atractylodes lances were studied with orthogonal design.Result] The culture medium suitable for the proliferation of the Atractylodes lances tube seedling was MS+6-BA 1.0 mg/L+NAA 0.4 mg/L+KT 0.4 mg/L.And the medium suitable for rooting was 1/2MS+NAA 0.5 mg/L.The optimal ratio of soil,riversand:cotton seed hull was 3∶3∶2.Conclusion] The research selected optimum culture medium,rooting medium and ratio of substrate,which provided technical guidance for laboratorial preservation of germplasm resource of Atractylodes lances and industrialization of its seedling production.