川产道地药材三叶黄连HPLC指纹图谱研究

目的]建立川产道地药材三叶黄连的指纹图谱检测标准。方法]以盐酸小檗碱为参照物,采用依利特SinoChrom ODS-BP(250mm×4.6 mm,5μm)色谱柱,以乙腈-浓度0.1%乙酸和10 mmol乙酸铵溶液为流动相梯度洗脱,流速为1.0 ml/min,检测波长为345nm。结果]药材有14个共有峰,共有峰的参数符合"中药注射剂指纹图谱技术要求"的有关规定。结论]该方法准确、重复性好,建立的指纹图谱检测标准,可作为川产道地药材三叶黄连质量评价与品种鉴别的重要依据之一。

Study on the HPLC Fingerprint of RHIZOMA COPTIDIS in Sichuan

Objective] To establish the fingerprint detecting standard for RHIZOMA COPTIDIS in Sichuan.Method] Using berberine hydrochloride as the reference substance,the separation was carried out on a SinoChrom ODS-BP(250 mm×4.6 mm,5μm) with the detection wavelength at 345 nm.Following the injection of sample solution,a gradient elution with methanol-0.1%caproic acid and 10 mmol/L ammonium acetate as mobile phase was initiated at a flow rate of 1.0 ml/min.Result] A total of 14 common peaks were found in the chromatogram of RHIZOMA COPTIDIS,the parameter of which met with the stipulation of Chinese Medicine Injection Fingerprint Technical Requirements.Conclusion] The method is simple and accurate with a good reproducibility and can be used in quality evaluation and variety discrimination of RHIZOMA COPTIDIS in Sichuan.