| HPLC法测定留兰香油中柠檬烯 |
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| 引用本文： | 张瑞伦,张庆,徐世艾.HPLC法测定留兰香油中柠檬烯[J].安徽农业科学,2012,40(2):743-744. |
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| 作者姓名： | 张瑞伦 张庆 徐世艾 |
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| 作者单位： | 山东省烟台大学,化学工程与过程重点试验室,化工制造工程高校重点试验室,山东烟台264005;山东省烟台大学,化学工程与过程重点试验室,化工制造工程高校重点试验室,山东烟台264005;山东省烟台大学,化学工程与过程重点试验室,化工制造工程高校重点试验室,山东烟台264005 |
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| 基金项目： | 国家自然科学基金资助项目，国家"863"重点资助项目，山东省自然科学基金重点资助项目 |
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| 摘 要： | 目的]建立高效液相色谱法测定留兰香油中柠檬烯的含量。方法]以无水甲醇为外标物,对留兰香油中柠檬烯的含量进行测定。色谱条件:色谱柱为C18(4.6 mm×250 mm,5μm),流动相为无水甲醇-水(75∶25,V/V),流速为1.0 ml/min,检测波长为200 nm,柱温为25℃。结果]柠檬烯含量在0.21～1.05 mg/ml时,其峰面积与浓度呈线性关系,相关系数R2为0.995,平均回收率为98.0%,RSD为2.6%;计算得留兰香油原料中柠檬烯的含量为24.2%。结论]该方法简单快速、准确,可用于留兰香油中柠檬烯的含量测定。
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| 关 键 词： | 留兰香(Mentha spicata L.) 柠檬烯 高效液相色谱 |
Determination of Limonene in Spearmint Oil by HPLC Method |
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| Institution: | ZHANG Rui-lun et al(Shandong Province Key Lab of Chemical Engineering and Process,Key Lab of Chemical Manufacture Engineering of Shandong Province Universities,Yantai University,Yantai,Shandong 264005) |
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| Abstract: | Objective] A HPLC method was established for the determination of limonene in spearmint oil.Methods] The limonene was quantitatively determined using Absolute methanol as external standard,separated on a C18 column(4.6 mm×250 mm,5 μm) using methanol-water(75∶25,V/V) as mobile phase at a flow rate of 1.0 ml/min.The UV detection wavelength was set at 200 nm and column temperature was 25 ℃ in HPLC.Results] The results showed that when the content of limonene were among 0.21-1.05 mg/ml,good linear dependence of peak area on concentration of limonene and the correlation coefficient R2 was 0.995,and the average recovery was 98.0%,RSD was 2.6%;after the calculation,the content of limonene in spearmint oil was 24.2%.Conclusion] The method was simple,rapid and precise for the analysis of limonene in spearmint oil. |
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| Keywords: | Spearmint(Mentha spicata L ) Limonene HPLC |
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