番茄幼苗根系总蛋白SDS-PAGE方法的优化 |
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引用本文: | 邢佳毅,雷慧,程继鸿,杨瑞,王绍辉.番茄幼苗根系总蛋白SDS-PAGE方法的优化[J].安徽农业科学,2012,40(2):675-677. |
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作者姓名: | 邢佳毅 雷慧 程继鸿 杨瑞 王绍辉 |
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作者单位: | 北京农学院植物科学技术学院,农业应用新技术北京市重点实验室,北京102206;北京农学院植物科学技术学院,农业应用新技术北京市重点实验室,北京102206;北京农学院植物科学技术学院,农业应用新技术北京市重点实验室,北京102206;北京农学院植物科学技术学院,农业应用新技术北京市重点实验室,北京102206;北京农学院植物科学技术学院,农业应用新技术北京市重点实验室,北京102206 |
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基金项目: | 教育部科学研究重点课题资助 |
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摘 要: | 目的]建立一套适合番茄幼苗根系蛋白质组分析的SDS-PAGE方法和获得清晰的电泳图谱。方法]以番茄幼苗根系为试材,对根系蛋白的提取方法和上样量等因素进行了优化。结果]上样量为60μg时,改良后的TCA-丙酮沉淀法提取的蛋白能够获得分辨率较高、图像清晰、重复性好的SDS-PAGE图谱。结论]该法可以基本满足番茄幼苗根系蛋白质组学的研究。
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关 键 词: | 番茄根系 蛋白组学 SDS-PAGE |
Optimization of SDS-PAGE Procedures for Analyzing the Total Protein In Seedling Roots of Lycopersicon esculentum |
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Institution: | XING Jia-yi et al (Beijing Key Laboratory of New Technology in Agricultural Application,College of Plant Science,Beijing University of Agriculture,Beijing 102206) |
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Abstract: | Objective] This study aimed to develop an efficient SDS-PAGE method suitable for analyzing the total protein of Lycopersicon esculentum seedling roots.Method] By using the L.esculentum seedling roots as the experimental material,the protein extraction,sample volume and other factors influencing the final electrophoresis pattern were respectively optimized.Result] The high-resolution,clear and reproducible SDS-PAGE pattern could be obtained when the protein was extracted with modified TCA-acetone precipitation and the sample volume for electrophoresis was 60 μg.Conclusion] This optimized method could basically meet the requirements for study on the proteome of tomato seedling roots. |
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Keywords: | Tomato roots Proteome SDS-PAGE |
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