| 东北矮紫杉细胞悬浮培养中褐化问题的研究 |
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| 引用本文： | 王培忠,赵欣,张宗申.东北矮紫杉细胞悬浮培养中褐化问题的研究[J].安徽农业科学,2012,40(10):5760-5762. |
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| 作者姓名： | 王培忠 赵欣 张宗申 |
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| 作者单位： | 大连工业大学生物工程学院,辽宁大连,116034;大连工业大学生物工程学院,辽宁大连,116034;大连工业大学生物工程学院,辽宁大连,116034 |
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| 基金项目： | 国家自然科学基金项目(31070164);大连市青年科技人才基金(2006J23JH031) |
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| 摘 要： | 目的]研究东北矮紫杉悬浮培养细胞褐化现象,为紫衫细胞悬浮培养提供指导方法。方法]以东北矮紫杉愈伤组织为试验材料,探讨不同培养基、N/P比、pH、培养转速、光照及强度等因素对矮紫杉细胞悬浮培养褐化的影响。结果]将无褐化的愈伤组织转接到pH 7.0的2mB5培养基中,于22℃、1 500 lx光强、90 r/min转速的条件下光照培养24 h,细胞褐化现象能得到明显抑制。结论]该研究结果为紫衫细胞悬浮培养奠定了基础,对于大规模工业化生产紫杉醇具有重要意义。
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| 关 键 词： | 东北矮紫杉 褐化 愈伤组织 悬浮细胞培养 |
Research on the Browning in Suspension Cell Culture of Taxus cuspidata |
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| Institution: | WANG Pei-zhong et al(College of Biological Engineering,Dalian Polytechnic University,Dalian,Liaoning 116034) |
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| Abstract: | Objective] This study aimed to investigate the browning of T.cuspidata cells in suspension culture and provide a guidance for the cell suspension culture of T.cuspidata.Method] T.cuspidata callus were used as experimental materials,to explore the effects of different medium,N/P ratio,pH,shaking speed,illumination time and light intensity and other factors on browning of T.cuspidata cells in suspension culture.Result] Non-browning callus were transferred to 2MB5 medium(pH 7.0) for light culture at 22 ℃ under light intensity of 1 500 lx with shaking speed of 90 r/min for 24 h.Results showed that the cell browning was significantly inhibited.Conclusion] This study laid the foundation for cell suspension culture of T.cuspidata and had important significance to the large-scale industrial production of paclitaxel. |
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| Keywords: | Taxus cuspidata Browning Callus Cell suspension culture |
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