豆豉纤溶酶产酶菌的筛选及菌种保藏

目的]从豆豉产品中筛选分离活性较高的豆豉纤溶酶产酶菌,并对菌种进行保藏。方法]用自制的猪血粉为底物配制筛选培养基进行豆豉纤溶酶产酶菌的筛选,然后根据水解圈的大小筛选活性高的产酶菌株,最后将其保藏于LB培养基中。结果]成功从湖南、广东、江西等地产的豆豉中筛选到一株溶栓活性相对较高的产酶菌。结论]该研究为新型溶栓药物的开发奠定了基础。

Screening and Preservation of Douchi Fibrinolytic Enzyme Producing Strain

Objective] To isolate and preserve a high-activity Douchi fibrinolytic enzyme producing strain from Douchi products.Method] The selection of Douchi fibrinolytic enzyme producing strain was carried out on the selected medium prepared with self-made pork blood powder,the strain with the highest activity was screened out according to the size of hydrolysis cycle,and then preserved in LB medium.Result] A Douchi fibrinolytic enzyme proudcing strain with high thrombolytic activity was screened out from the Douchi produced in Hunan,Guangdong and Jiangxi Provinces.Conclusion] The study laid foundation for the development of new-type thrombolytic drugs.