| 产甲烷细菌保藏方法研究 |
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| 引用本文： | 张万芹,康冀川,王开功. 产甲烷细菌保藏方法研究[J]. 安徽农业科学, 2012, 40(10): 5775+5778 |
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| 作者姓名： | 张万芹 康冀川 王开功 |
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| 作者单位： | 兴以民族师范学院,贵州兴义,562400;贵州大学贵州省生化工程中心,贵州贵阳,550025;贵州大学动物科学学院,贵州贵阳,550025 |
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| 基金项目： | 贵州省教育厅自然科学研究资助项目(20090100) |
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| 摘 要： | [目的]研究适合产甲烷细菌保存的方法。[方法]采用严格厌氧技术,对从贵阳周边沼气池中初步筛选的28株产甲烷细菌进行保存,经过短期、长期保存方法试验,测得短期保藏6个月和12个月后,菌株存活率为71.4%,18个月为67%;长期保藏18个月菌株存活率为57.1%。[结论]结果为重新开展产甲烷细菌保藏方法的研究奠定了基础。
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| 关 键 词： | 产甲烷细菌 保藏方法 存活率 |
Study on the Preservation Method for Methanogenic Bacteria |
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| Affiliation: | ZHANG Wan-qin et al(Xingyi Normal University for Nationalities,Xingyi,Guizhou 562400) |
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| Abstract: | [Objective] The aim was to study the preservation method for methanogenic bacteria.[Method] 28 strains of methanogenic bacteria which preliminarily screened from Guiyang peripheral methane tank were preserved.[Result] The results showed that the survival rates were 71.4% after 6 and 12 months,67% after 18 months for short-term preservation;after 18 months for long-term preservation,the survival rate was 57.1%.[Conclusion] The results lay the foundation for restudying the preservation method for methanogenic bacteria. |
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| Keywords: | Methanogenic bacteria Preservation method Survival rate |
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