| 一种广泛适用于植物基因组DNA提取的方法 |
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| 引用本文： | 李斌,于丽霞,习珺珺,张亚萍,高则睿,鄢波. 一种广泛适用于植物基因组DNA提取的方法[J]. 安徽农业科学, 2012, 40(10): 5806-5807 |
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| 作者姓名： | 李斌 于丽霞 习珺珺 张亚萍 高则睿 鄢波 |
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| 作者单位： | 1. 西南林业大学,云南昆明,650222
2. 云南烟草科学研究院,云南昆明,650106 |
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| 基金项目： | 国家自然科学基金(31060042);云南省应用基础研究项目资助(2009ZC079M) |
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| 摘 要： | [目的]研究植物基因组DNA提取方法,为从植物组织中快速提取基因组DNA提供指导。[方法]在已发表的植物基因组DNA提取方法基础上,对SDS提取法的操作步骤、试剂用量及作用时间进行了优化。[结果]初步建立了一种广泛适用于各类植物及植物多种组织器官的基因组DNA提取方法。所提取的基因组DNA质量较好,满足下一步操作的要求。[结论]该研究结果为植物基因组DNA的快速提取提供了指导方法。
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| 关 键 词： | 植物基因组 DNA提取 优化SDS提取法 |
A Widely Applicable Method for Plant Genomic DNA Extraction |
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| Affiliation: | LI Bin et al(Southwest Forestry University,Kunming,Yunnan 650222) |
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| Abstract: | [Objective] This study aimed to explore a method for plant genomic DNA extraction,to provide guidance for rapid extraction of genomic DNA from plant tissues.[Method] Based on the published genomic DNA extraction methods,operation steps,reagent amount and processing time of SDS extraction method were optimized.[Result] A widely applicable method was established initially for genomic DNA extraction from various varieties of plants and various kinds of plant tissues.Quality of the extracted genomic DNA was relatively good,which met the requirements for further operation.[Conclusion] This study provided guidance for rapid plant genomic DNA extraction. |
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| Keywords: | Plant genome DNA extraction Optimized SDS extraction method |
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