稀土元素镧对Corynebacterium pekinense发酵赖氨酸的影响

[目的]研究稀土元素镧对北京棒杆菌(Corynebacterium pekinense)的生长及产物合成的影响。[方法]通过全因子试验设计,分析了在北京棒杆菌的发酵培养基中添加不同配比稀土元素镧对其赖氨酸产量的影响。[结果]低剂量的稀土元素镧可以促进北京棒杆菌生长并积累代谢产物赖氨酸,利用Matlab软件优化培养基中显著影响成分,得到稀土镧离子的最佳浓度为78 mg/L,葡萄糖为36 g/L,牛肉膏为4.5 g/L,蛋白胨为20 g/L。[结论]该研究初步确定了在北京棒杆菌培养中,La+的适宜添加量及该菌产生赖氨酸的最适培养基组成。

Effect of Lanthanon on Lysine Fermentation in Corynebacterium pekinense

[Objective] This study aimed to investigate the effects of rare earth element lanthanon on the growth and product synthesis of Corynebacterium pekinense.[Method] A full factorial experimental was designed to compare the lysine fermentation of C.pekinense in medium containing different concentrations of lanthanon.[Result] Low doses of lanthanum can promote the growth of C.pekinense and accumulation of metabolite lysine;optimization for distinct components of medium was obtained as rare earth 78 mg/L,glucose 36 g/L,beef extract 4.5 g/L,peptone 20 g/L by Matlab software.[Conclusion] The study initially determined the optimum La+ additive amount in C.pekinense culture medium and the optimum medium compositions for C.pekinense to produce lysine.