| 铜污染土壤微生物群落结构及酶活性研究 |
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| 作者姓名： | GE Chao-Rong ZHANG Qi-Chun |
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| 作者单位： | GE Chao-Rong(Laboratory of Clinical Microbiology, The First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou 310003,China);ZHANG Qi-Chun(Zhejiang Provincial Key Laboratory of Subtropic Soil and Plant Nutrition, Zhejiang University, Hangzhou 310029,China) |
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| 基金项目： | Supported by the Fundamental Research Funds for the Central Universities of China; the Zhejiang Provincial Natural Science Foundation of China (No. Y3080145) |
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| 摘 要： | The microbial community structure and enzyme activities of seven paddy soils with different Cu concentrations were investigated in the vicinity of a Cu smelter in Fuyang County,Zhejiang Province in Southeast China.The microbial community structure was analyzed using the phospholipid fatty acid (PLFA) and multiplex-terminal restriction fragment length polymorphism (M-TRFLP) techniques.There was no clear dose-response relationship between Cu pollution and soil enzyme activity except for urease.Both PLFA and M-TRFLP methods showed that Cu contamination had a large effect on the soil microbial community structure.PLFA indicators of Gram-positive bacteria (16:0i,15:0i) and fungi (18:2w6,9) relatively decreased with increasing Cu concentration,whereas indicators of Gram-negative bacteria (19:0cy,16:1w7) increased.The M-TRFLP results suggested that there was a dose-dependent response between Cu pollution and bacterial community or fungal community.The fungal community was more sensitive to Cu pollution than the bacterial community.There were no significant differences in archaeal community structure between the different Cu pollution plots and archaea might be more tolerant to Cu pollution than both bacteria and fungi.
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| 关 键 词： | archaea bacteria fungi multiplex-terminal restriction fragment length polymorphism phospholipid fatty acid |
Microbial community structure and enzyme activities in a sequence of copper-polluted soils |
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| GE Chao-Rong,ZHANG Qi-Chun.Microbial community structure and enzyme activities in a sequence of copper-polluted soils[J].Pedosphere,2011,21(2):164-169. |
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| Authors: | GE Chao-Rong and ZHANG Qi-Chun |
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| Institution: | Laboratory of Clinical Microbiology, The First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou 310003 (China);Zhejiang Provincial Key Laboratory of Subtropic Soil and Plant Nutrition, Zhejiang University, Hangzhou 310029 (China) |
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| Abstract: | The microbial community structure and enzyme activities of seven paddy soils with different Cu concentrations were investigated in the vicinity of a Cu smelter in Fuyang County, Zhejiang Province in Southeast China. The microbial community structure was analyzed using the phospholipid fatty acid (PLFA) and multiplex-terminal restriction fragment length polymorphism (M-TRFLP) techniques. There was no clear dose-response relationship between Cu pollution and soil enzyme activity except for urease. Both PLFA and M-TRFLP methods showed that Cu contamination had a large effect on the soil microbial community structure. PLFA indicators of gram-positive bacteria (16:0i, 15:0i) and fungi (18:2w6,9) relatively decreased with increasing Cu concentration, whereas indicators of gram-negative bacteria (19:0cy, 16:1w7) increased. The M-TRFLP results suggested that there was a dose-dependent response between Cu pollution and bacterial community or fungal community. The fungal community was more sensitive to Cu pollution than the bacterial community. There were no significant differences in archaeal community structure between the different Cu pollution plots and archaea might be more tolerant to Cu pollution than both bacteria and fungi. |
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| Keywords: | archaea bacteria fungi multiplex-terminal restriction fragment length polymorphism phospholipid fatty acid |
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