山羊成纤维细胞体外培养研究

分别采用组织块法与消化法对山羊耳成纤维细胞进行原代培养,比较两种方法的培养效果.结果表明,组织块法培养的细胞生长稳定,5～7d有细胞生长,15～18d长满单层;消化法培养的细胞,通过简化酶作用时间及用量的调控,可得到较为单一的成纤维细胞,且经过4～7d生长后能形成单层.同时对细胞培养过程中常遇到的污染问题及解决方法进行了探讨,为动物组织培养研究提供了实验依据.

Study on in vitro culture of goat fibroblast cells

By the method of tissue explants and digesting culture, the ear fibroblast cell of goat was cultured to comparing the effect of two culture methods. The results indicated that the cells in tissue culturing grew stable, the cells were found after 5-7 days, and the monolayer overgrew after 15-18days. The single fibroblast cells cultured by digesting were obtained by reducing the enzyme action time and controlling its dosage, and the monolayer was obtained after 4-7 days. Moreover, the ordinary polluted problems and its resolution in the process of cell culture were discussed to provide the basis on studying the tissue culture of animal.