| 桑枝中氧化白藜芦醇的分离与鉴定 |
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| 引用本文： | 佟志远,颜新培,李顺祥,周晋,蔡光先,郑群怡,黄丹.桑枝中氧化白藜芦醇的分离与鉴定[J].蚕业科学,2011,37(5):948-951. |
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| 作者姓名： | 佟志远 颜新培 李顺祥 周晋 蔡光先 郑群怡 黄丹 |
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| 作者单位： | 湖南中医药大学药学院,长沙410208;湖南省中医药研究院,中药新药研究与开发省重点实验室,长沙410013;湖南省蚕桑科学研究所,长沙,410127;湖南中医药大学药学院,长沙,410208;蕾硕医药化工长沙有限公司,长沙,410329 |
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| 基金项目： | 湖南省科技计划重大专项(No.2010FJ1010-3); 湖南省教育厅重点课题(No.10A091); 国家中医药管理局“中药植物学”重点学科课题(No.国中医药发[2009]30号); 湖南省高校科技创新团队资助项目(No.湘教通[2010]212号) |
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| 摘 要： | 氧化白藜芦醇具有抑制酪氨酸酶活性的作用。为了从桑枝中有效提取这一生物活性物质,采用乙醇提取法获得桑枝粗提液,通过硅胶柱、葡聚糖柱柱层析法分离制备桑枝氧化白藜芦醇样品。运用核磁共振、HPLC-MS等方法鉴定样品的化学结构和分子质量,证实制备样品为氧化白藜芦醇,通过高效液相色谱检测其质量分数大于99%。研究结果为从桑枝中分离提取氧化白藜芦醇提供了一种简便有效的方法。
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| 关 键 词： | 氧化白藜芦醇 桑枝 分离 纯化 |
Isolation and Identification of Oxyresveratrol in Mulberry Branch |
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| TONG Zhi-Yuan,YAN Xin-Pei,LI Shun-Xiang,ZHOU Jin,CAI Guang-Xian,ZHENG Qun-Yi,HUANG Dan.Isolation and Identification of Oxyresveratrol in Mulberry Branch[J].Acta Sericologica Sinica,2011,37(5):948-951. |
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| Authors: | TONG Zhi-Yuan YAN Xin-Pei LI Shun-Xiang ZHOU Jin CAI Guang-Xian ZHENG Qun-Yi HUANG Dan |
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| Institution: | TONG Zhi-Yuan1,2 YAN Xin-Pei3* LI Shun-Xiang1,2* ZHOU Jin1 CAI Guang-Xian1,2 ZHENG Qun-Yi4 HUANG Dan1,2(1School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China,2Key Provincial Lab of Novel Chinese Medicine Research and Development,Hunan Academy of Chinese Medicine,Changsha 410013,3The Sercultural Scientific Research Institute of Hunan Province,Changsha 410127,4Natsource Chemicals Changsha Corporation,Changsha 410329,China) |
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| Abstract: | Oxyresveratrol has the inhibitory activity on tyrosinase.In order to extract this bioactive substance from mulberry branch,ethanol fractionation,silica gel column chromatography and Sephadex LH-20 gel filtration chromatography were employed to isolate and prepare mulberry oxyresvertrol sample.Identification of its chemical structure and molecular weight by nuclear magnetic resonance(NMR),HPLC-MS and other measures verified that the prepared sample was oxyresvertrol.Detection with high performance liquid chr... |
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| Keywords: | Oxyresveratrol Mulberry branch Isolation Purification |
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