猪正反交F1代背最长肌中H3.3基因表达的发育性变化

为了解猪组蛋白变异体H3.3基因在组织中表达的发育性变化规律,本研究通过实时荧光定量PCR的方法,检测H3.3基因在不同日龄和正反交组合下在猪肌肉组织中的表达水平。试验结果表明,猪H3.3基因在猪出生后各个时期的背最长肌中均有表达,且随日龄的增加表达量也随之增加;正反交间表达量也有所差异,1日龄反交(二花脸猪♂×大约克猪♀)组表达量大于正交(大约克猪♂×二花脸猪♀)组,20日龄以后,均为正交组表达量高于反交组,但同时期各组正反交表达量间均差异不显著。本试验首次证实了组蛋白变异体H3.3在猪肌肉组织中确有掺入,得到了H3.3在猪肌细胞中表达量的发育性规律,并且认为H3.3的替换作用可能受到母体效应的影响。本研究为更深入的研究组蛋白变体的替换对真核生物细胞生长和发育相关基因的调控与修饰分子机制的研究奠定基础。

Developmental Changes of the H3.3 Gene Expression in Longissimus Muscle of F1 Generation of Reciprocal Cross Pigs

To understand the developmental rules of histone variant H3.3 gene expression of pigs, we detected the expressional level of swine muscle issue under different ages and reciprocal cross. The rusults showed that the H3.3 gene were expressed in longissimus muscle in different periods after birth and the expression substantially growed in number with increasing age. There was difference between reciprocal cross pigs: anti-cross (Erhualian♂×Yorkshire♀) group of one-day age expressed greater than the orthogonal (Yorkshire♂×Erhualian♀) group;expression of the orthogonal group were higher than the anti-cross group after 20 days, while there was no significant difference in expression of orthogonal group and anti-cross group uder the same period. Our study confirmed histone variant H3.3 incoorporate in the swine muscle issue and obtain developmental rules of H3.3 expression in muscle cells and to further study the replacement of histione variants on eukaryotic cell growth and development-related gene regulation and modification of basis for the study of molecular mechanisms.