| 一种引起成年桑树根部腐烂的病害及致病菌分离与初步鉴定 |
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| 引用本文： | 方水琴,吴福安,陈明胜,陶恒平,梁垚,姜星,程嘉翎,韩红发,张洁花. 一种引起成年桑树根部腐烂的病害及致病菌分离与初步鉴定[J]. 蚕业科学, 2011, 37(5): 785-791 |
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| 作者姓名： | 方水琴 吴福安 陈明胜 陶恒平 梁垚 姜星 程嘉翎 韩红发 张洁花 |
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| 作者单位： | 江苏科技大学生物与环境工程学院,江苏镇江,212018;江苏科技大学生物与环境工程学院,江苏镇江212018;中国农业科学院蚕业研究所,江苏镇江212018;山西省蚕业科学研究院,山西运城,044000 |
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| 基金项目： | 现代农业产业技术体系专项(No.CARS-22) |
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| 摘 要： | 对山西省运城市投产桑园内桑树发生根部腐烂、枝叶萎凋枯死病害的病原菌进行分离鉴定,为病害的有效控制提供理论依据。从桑树发病植株根部分离到一株病原菌(暂命名为TY.GF1),将该病原菌接种到健康桑树根部,其致病症状与桑根腐病的症状相似。该病原菌在PDA培养基上的培养性状及孢子形态特征与米根霉(Rhizopus oryzae)相似。通过病原菌TY.GF1的核糖体18S rDNA及内部转录间隔区(ITS)序列PCR扩增和测序,分别获得长度为1 805、627 bp的序列片段。基于18S rDNA及ITS序列构建的进化树显示,与TY.GF1菌株亲缘关系最近的为米根霉和淀粉霉(Amylomyces rouxii)。结合病原菌致病性特点及形态特征与18S rDNA、ITS序列分析结果,初步鉴定这种新发生的桑树病害为桑根腐病,其病原菌可能为米根霉。
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| 关 键 词： | 桑树病害 桑根腐病 米根霉 致病性 18S rDNA 内部转录间隔区 系统进化 |
Isolation and Primary Identification of the Pathogen Causing Root Rot Disease of Adult Mulberry Trees |
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| FANG Shui-Qin,WU Fu-An,CHEN Ming-Sheng,TAO Heng-Ping,LIANG Yao,JIANG Xing,CHENG Jia-Ling,HAN Hong-Fa,ZHANG Jie-Hua. Isolation and Primary Identification of the Pathogen Causing Root Rot Disease of Adult Mulberry Trees[J]. Acta Sericologica Sinica, 2011, 37(5): 785-791 |
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| Authors: | FANG Shui-Qin WU Fu-An CHEN Ming-Sheng TAO Heng-Ping LIANG Yao JIANG Xing CHENG Jia-Ling HAN Hong-Fa ZHANG Jie-Hua |
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| Affiliation: | FANG Shui-Qin1 WU Fu-An1,2* CHEN Ming-Sheng1,2 TAO Heng-Ping1 LIANG Yao1 JIANG Xing1CHENG Jia-Ling1,2 HAN Hong-Fa3 ZHANG Jie-Hua3(1School of Biological and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China,2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,3The Sericultural Research Institute of Shanxi,Yuncheng Shanxi 044000,China) |
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| Abstract: | The pathogens causing mulberry root rot disease and leaf withering disease in Yuncheng city of Shanxi province were isolated and identified to provide theoretical basis for effective prevention and control of these diseases.A pathogenic fungal strain,designated as TY.GF1,was isolated from diseased mulberry root and inoculated into healthy mulberry root.The resultant symptom was similar to that of mulberry rhizopus rot.Appearance and spore morphological characters of the pathogen cultured on potato dextrose ... |
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| Keywords: | Mulberry disease Root rot disease Rhizopus oryzae Pathogenicity 18S rDNA Internal transcribed spacer Phylogenetic evolution |
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