| 猪圆环病毒2型不同地区毒株的分离鉴定及基因型分析 |
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| 引用本文： | 王贵华,陈义锋,刘明明,陈翠云,赵亚荣. 猪圆环病毒2型不同地区毒株的分离鉴定及基因型分析[J]. 动物医学进展, 2011, 0(10): 75-78 |
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| 作者姓名： | 王贵华 陈义锋 刘明明 陈翠云 赵亚荣 |
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| 作者单位： | 北京大北农动物医学研究中心; |
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| 摘 要： | 分别对收集于山东、山西、北京和河北的PCV-2核酸阳性的病料进行PCV-2分离鉴定,采用PCR和间接免疫荧光法对各分离毒株进行鉴定,并对各分离毒株的全基因组进行克隆、测序和基因型分析.结果共分离到5株PCV-2毒株,分别命名为DBN-SD02、DBN-SX07 DBN-BJ08、DBN-HB12和DBN-HB13株,...
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| 关 键 词： | 猪圆环病毒2型 分离 基因型 鉴定 |
Isolation,Identification and Genotype Analysis of Porcine Circovirus Type 2 Strains from Different Area |
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| WANG Gui-hua,CHEN Yi-feng,LIU Ming-ming,CHEN Cui-yun,ZHAO Ya-rong. Isolation,Identification and Genotype Analysis of Porcine Circovirus Type 2 Strains from Different Area[J]. Progress In Veterinary Medicine, 2011, 0(10): 75-78 |
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| Authors: | WANG Gui-hua CHEN Yi-feng LIU Ming-ming CHEN Cui-yun ZHAO Ya-rong |
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| Affiliation: | WANG Gui-hua,CHEN Yi-feng,LIU Ming-ming,CHEN Cui-yun,ZHAO Ya-rong(Veterinary Medicine Research Center of Beijing Da Bei Nong Group,Beijing,100097,China) |
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| Abstract: | To isolate porcine circovirus type 2 strains,and to identify its' genotype,polymerase chain reaction and indirect immunofluorescence assay were utilized in this study to detect and isolate PCV-2 from the specimens of PCV-2 DNA positive collected from Shandong,Shanxi,Beijing and Hebei province.Furthermore,the genome of each isolates was then cloned and sequenced,and the genotype of each isolate was identified based on the genome sequence.Five strains of PCV-2 were isolated and identified,named PCV-2 DBN-SD02... |
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| Keywords: | Porcine circovirus type 2 isolation genotype identification |
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