| 成年母猪后肠木聚糖降解菌的筛选、鉴定及其对饲草纤维降解作用的研究 |
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| 引用本文： | 董杰丽,张铁鹰,齐志国,吴兴群,欧阳张智. 成年母猪后肠木聚糖降解菌的筛选、鉴定及其对饲草纤维降解作用的研究[J]. 动物营养学报, 2011, 0(11): 1953-1960 |
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| 作者姓名： | 董杰丽 张铁鹰 齐志国 吴兴群 欧阳张智 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室;南京农业大学动物科技学院;西南民族大学生命科学与技术学院; |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金项目“成年猪后肠厌氧菌木聚糖酶基因克隆及其异源表达的研究”(2010jc-5-2); 公益性行业(农业)科研专项经费专项“奶牛良种扩繁技术与产业化开发”(nyhyzx07-036) |
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| 摘 要： | 本研究旨在通过对内蒙古黑猪成年母猪后肠木聚糖降解菌的分离、筛选及鉴定,并对比研究筛选菌和瘤胃液对饲草纤维降解的作用,获得可高效降解木聚糖的菌株.以木聚糖为唯一碳源,根据筛选平板上菌株透明圈大小和发酵液中木聚糖酶活性分离得到了2株活性较高的木聚糖降解菌(分别命名为ZF和MZ).经形态学观察和16S rDNA核苷酸序列分析...
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| 关 键 词： | 成年母猪 木聚糖降解菌 筛选 鉴定 纤维降解 |
Xylan-Degrading Bacteria from the Hindgut of Adult Sows: Screening,Identification and Effects on Degradation of Forage Fiber |
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| DONG Jieli ZHANG Tieying QI Zhiguo WU Xingqun OUYANG Zhangzhi. Xylan-Degrading Bacteria from the Hindgut of Adult Sows: Screening,Identification and Effects on Degradation of Forage Fiber[J]. CHINESE JOURNAL OF ANIMAL NUTRITION, 2011, 0(11): 1953-1960 |
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| Authors: | DONG Jieli ZHANG Tieying QI Zhiguo WU Xingqun OUYANG Zhangzhi |
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| Affiliation: | DONG Jieli1 ZHANG Tieying1* QI Zhiguo1 WU Xingqun2 OUYANG Zhangzhi3(1.State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China,2.College of Animal Science,Nanjing Agriculture University,Nanjing 210095,3.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China) |
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| Abstract: | This experiment was conducted to study the isolation,screening and identification of efficient xylan-degrading bacteria from the hindgut of high roughage-resistance Inner Mongolia black adult sows,and to compare the effects of screening bacteria and rumen liquid on neutral detergent fiber(NDF) and acid detergent fiber(ADF) degradation of forages(alfalfa,Chinese wildrye and silage corn).Using xylan as the sole carbon source,two xylan-degrading bacteria named ZF and MZ were isolated by screening with the diam... |
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| Keywords: | adult sows xylan-degrading bacteria screening identification fiber degradation |
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