| 旋毛虫成虫丝氨酸蛋白酶对S774A.1巨噬细胞的毒性作用 |
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| 引用本文： | 于建立,白雪,刘国兴,吴秀萍,王学林,刘明远. 旋毛虫成虫丝氨酸蛋白酶对S774A.1巨噬细胞的毒性作用[J]. 动物医学进展, 2011, 0(10): 5-9 |
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| 作者姓名： | 于建立 白雪 刘国兴 吴秀萍 王学林 刘明远 |
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| 作者单位： | 吉林大学人兽共患病研究所;吉林大学畜牧兽医学院; |
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| 基金项目： | 国家自然科学基金项目(31030064); 国家杰出青年科学基金项目(30825033) |
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| 摘 要： | 为探究旋毛虫成虫排泄分泌抗原(ES)中丝氨酸蛋白酶对宿主免疫调节作用,用PCR扩增出旋毛虫成虫期特异性丝氨酸蛋白酶基因Zh68,克隆至表达载体pET28a,转化到大肠埃希菌BL21( DE3),经诱导表达后的重组蛋白免疫接种动物获取抗血清.将抗体封闭前后的ES分别作用于S774A.1巨噬细胞,CCK-8检测巨噬细胞的增...
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| 关 键 词： | 旋毛虫成虫 排泄分泌抗原 丝氨酸蛋白酶 巨噬细胞 毒性作用 |
Toxicity of Serine Ptotease of Adult Trichinella spiralis on S774A.1 Macrophages |
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| YU Jian-li,BAI Xue,LIU Guo-xing,WU Xiu-ping,WANG Xue-lin,LIU Ming-yuan. Toxicity of Serine Ptotease of Adult Trichinella spiralis on S774A.1 Macrophages[J]. Progress In Veterinary Medicine, 2011, 0(10): 5-9 |
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| Authors: | YU Jian-li BAI Xue LIU Guo-xing WU Xiu-ping WANG Xue-lin LIU Ming-yuan |
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| Affiliation: | YU Jian-li,BAI Xue,LIU Guo-xing,WU Xiu-ping,WANG Xue-lin,LIU Ming-yuan(Institute of Zoonoses,College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin,130062,China) |
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| Abstract: | To investigate the effect of serine protease in excretory-secretory antigens(ES) antigens of adult Trichinella spiralis on the host's immune system,full length sequence of serine protease gene Zh68 was amplified by PCR.The PCR products was cloned into pET28a vector,and the positive recombinant plasmid was transformed into Escherichia coli BL21(DE3).The Zh68 antibody was acquired form the rabbit by immunization with recombinant Zh68 protein.ES or ES treated by Zh68 antibody were incubated with S774A.1 macrop... |
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| Keywords: | adult Trichinalle spiralis excretory-secretory antigen serine protease macrophage toxicity |
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