| 伪狂犬病病毒主要抗原表位基因KgE的表达及纯化 |
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| 引用本文： | 周松峰,吴健敏,关忠宜,廖文军,白安斌.伪狂犬病病毒主要抗原表位基因KgE的表达及纯化[J].动物医学进展,2011,32(11):6-9. |
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| 作者姓名： | 周松峰 吴健敏 关忠宜 廖文军 白安斌 |
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| 作者单位： | 1. 广西大学动物科学技术学院,广西南宁530005;广西兽医研究所,广西南宁530001
2. 广西兽医研究所,广西南宁,530001 |
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| 基金项目： | 农业科技跨越计划项目(2009跨21); 广西自然科学基金项目(2011GXNSFA018086) |
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| 摘 要： | 从伪狂犬病病毒(PRV)基因组中克隆出KgE抗原表位基因,并将其插入到原核表达载体pETTrx中,构建了原核表达质粒pET-Trx-KgE,将pET-Trx-KgE转化至BL21 (DE3) plysS中,在IPTG诱导下进行表达.SDS-PAGE分析表明,KgE基因在BL21( DE3) plysS中获得高效表达,表...
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| 关 键 词： | 伪狂犬病病毒 KgE基因 原核表达 |
Expression and Purification of Pseudorabies Virus Main Antigen Epitope Gene KgE |
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| ZHOU Song-feng,WU Jian-min,GUAN Zhong-yi,LIAO Wen-jun,BAI An-bin.Expression and Purification of Pseudorabies Virus Main Antigen Epitope Gene KgE[J].Progress In Veterinary Medicine,2011,32(11):6-9. |
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| Authors: | ZHOU Song-feng WU Jian-min GUAN Zhong-yi LIAO Wen-jun BAI An-bin |
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| Institution: | ZHOU Song-feng1,2,WU Jian-min2,GUAN Zhong-yi2,LIAO Wen-jun2,BAI An-bin2 (1.College of Animal Sciences and Technology,Guangxi Univeristy,Nanning,Guangxi,530005,China,2.Guangxi Veterinary Research Institute,530001,China) |
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| Abstract: | The main antigen epitope gene KgE was cloned from pig pseudorabies virus genome by conventional PCR method and inserted into the prokaryotic expression vector pET-trx,constructed recombinant plasmid pET-Trx-KgE and then transformed into competent cell BL21.The target protein was expressed with IPDG induction and obtained a high level expression by SDS-PAGE analysis,it took possession of whole bacterial proteins about 32.8% and presented as inclusion bodies mainly with weight 36 ku.The purity of expressed pr... |
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| Keywords: | Pseudorabies virus KgE gene prokaryotic expression |
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