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| 口蹄疫病毒抗原决定簇融合基因转化苜蓿 | |
| 引用本文： | 王鸣刚,罗茂春,彭轶楠,芦真,陈亮. 口蹄疫病毒抗原决定簇融合基因转化苜蓿[J]. 草业科学, 2011, 28(8): 1546-1551 |
| 作者姓名： | 王鸣刚 罗茂春 彭轶楠 芦真 陈亮 |
| 作者单位： | 1. 兰州理工大学生命科学与工程学院,甘肃兰州,730050 2. 龙岩学院生物系,福建龙岩,364012 3. 厦门大学生命科学学院,福建厦门,361005 |
| 基金项目： | 甘肃省教育厅研究生导师基金(0703-11); 兰州理工大学博士基金(SB08200602) |
| 摘 要： | 摘要：通过农杆菌的介导，以携带O型和A型口蹄疫抗原决定簇融合基因O21 O14 A21 HBcAg的植物表达载体转化苜蓿(Medicago sativa)愈伤组织，并再生得到抗性植株。试验建立了苜蓿甘农1号愈伤组织再生系统；优化了农杆菌介导的苜蓿遗传转化系统；获得抗性再生植株4棵；GUS报告基因在转化的愈伤组织中得到瞬时表达；抗性植株PCR检测结果证实目的基因已经成功整合到苜蓿基因组中。 |
| 关 键 词： | 口蹄疫抗原决定簇；遗传转化；苜蓿 |
A study on transformation of fused gene O_(21)-O_(14)-A_(21)-HBcAg of FMDV epitopes into Medicago sativa |
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| WANG Ming-gang,LUO Mao-chun,PENG Yi-nan,LU Zhen,CHEN Liang. A study on transformation of fused gene O_(21)-O_(14)-A_(21)-HBcAg of FMDV epitopes into Medicago sativa[J]. Pratacultural Science, 2011, 28(8): 1546-1551 | |
| Authors: | WANG Ming-gang LUO Mao-chun PENG Yi-nan LU Zhen CHEN Liang |
| Affiliation: | WANG Ming-gang1,LUO Mao-chun2,PENG Yi-nan1,LU Zhen3,CHEN Liang3(1.School of Life Science and Engineering,Lanzhou University of Technology,Gansu Lanzhou 730050,China,2.Biological department,college of Longyan,Fujian Longyan 364012,3.School of Life Science,Xiamen University,Fujian Xiamen 361005,China) |
| Abstract: | The fused gene O21-O14-A21-HBcAg that contained both type O and A of FMDV epitopes were introduced into alfalfa(Medicago sativa cv.Gannong No.1)leaf explants via Agrobacterium tumefaciens-mediated transformation.After infection and co-culture resistant plants were obtained from the resistant callus and the genetic transformation and regeneration system of alfalfa were established.The GUS reporter gene was transitorily expressed in transformed callus.52 regenerated individual plants were acquired through Hyg... |
| Keywords: | FMDV antigenic determinant genetic transformation Medicago sativa |
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