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| 奶牛ACC基因SYBRGreenⅠ荧光定量PCR检测方法的建立 | |
| 引用本文： | 李鹏,刘敏跃,郭洋,刘国文,王哲. 奶牛ACC基因SYBRGreenⅠ荧光定量PCR检测方法的建立[J]. 饲料工业, 2011, 32(21) |
| 作者姓名： | 李鹏 刘敏跃 郭洋 刘国文 王哲 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,沈阳农业大学畜牧兽医学院,130062,吉林长春 2. 辽宁省农牧业机械研究所有限公司 3. 沈阳农业大学畜牧兽医学院 4. 吉林大学畜牧兽医学院 |
| 基金项目： | 国家自然科学基金项目[30871897] |
| 摘 要： | 乙酰辅酶A羧化酶(ACC)在奶牛肝脏中脂肪酸的合成及氧化代谢过程中起着关键作用,ACC mRNA的表达水平对于研究奶牛酮病、脂肪肝等物质代谢障碍性疾病具有重要意义。研究采用双标准曲线法,成功建立了奶牛ACC基因荧光定量检测方法。结果表明,建立的SYBR Green I荧光定量PCR方法具有操作简便、特异性强、定量准确等优点,为进一步对ACC基因定量分析,研究其在奶牛酮病、脂肪肝等营养代谢性疾病中的作用奠定了基础。 |
| 关 键 词： | 奶牛 ACC基因 荧光定量PCR |
Establishment of fluorescence quantitative PCR for detecting ACC gene |
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| Li Peng,Liu Minyue,Guo Yang,Liu Guowen,Wang Zhe. Establishment of fluorescence quantitative PCR for detecting ACC gene[J]. Feed Industry, 2011, 32(21) | |
| Authors: | Li Peng Liu Minyue Guo Yang Liu Guowen Wang Zhe |
| Affiliation: | Li Peng,Liu Minyue,Guo Yang,Liu Guowen,Wang Zhe |
| Abstract: | Acetyl-CoA carboxylase(ACC) catalyzes the carboxylation of acetyl-CoA to produce malonyl-CoA.ACC is an important enzyme of regulating fatty acid synthesis and oxidation metabolism in liver of dairy cow.Two standard curves were applied in this experiment and fluorescence quantitative PCR method was estabilished for detecting ACC.The results showed that the method of SYBRGreen Ⅰ FQ-PCR for detecting the expression of ACC mRNA was established successfully and provided useful methodological basis for quantitative analysis of ACC gene in dairy cow. |
| Keywords: | cow ACC gene fluorescence quantitative PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |