| 两株犬源贾第虫tpi基因的基因型分析 |
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| 引用本文： | 张萍,朱海波,李结,李金平,刘远佳,郭建超,孟祥龙,李国清.两株犬源贾第虫tpi基因的基因型分析[J].中国畜牧兽医,2011,38(10):59-62. |
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| 作者姓名： | 张萍 朱海波 李结 李金平 刘远佳 郭建超 孟祥龙 李国清 |
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| 作者单位： | 华南农业大学兽医学院,广东广州 510642 |
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| 基金项目： | 国家自然科学基金项目(30972179) |
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| 摘 要： | 采用巢式PCR鉴定广东两株犬源贾第虫(GD1和GD2)的基因型。运用碘液染色对犬粪便中贾第虫进行鉴定,提取DNA后经巢式PCR扩增tpi基因,扩增产物测序后用BLAST和DNAStar软件进行同源性和系统发育分析。结果表明,通过tpi基因分析,GD1与L02120的同源性为100%,种系发育树上位于同一分支;GD2与DQ220289的同源性为99.1％,种系发育树上两者在同一分支。广东犬源贾第虫GD1为人畜共患的A1型,GD2为犬的D型。
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| 关 键 词： | 犬 贾第虫 tpi基因 巢式PCR |
| 收稿时间： | 2011-03-24 |
Genotyping of Two Strains of Giardia from Dogs by tpi Gene |
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| ZHANG Ping,ZHU Hai-bo,LI Jie,LI Jin-ping,LIU Yuan-jia,GUO Jian-chao,MENG Xiang-long,LI Guo-qing.Genotyping of Two Strains of Giardia from Dogs by tpi Gene[J].China Animal Husbandry & Veterinary Medicine,2011,38(10):59-62. |
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| Authors: | ZHANG Ping ZHU Hai-bo LI Jie LI Jin-ping LIU Yuan-jia GUO Jian-chao MENG Xiang-long LI Guo-qing |
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| Institution: | College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642, China |
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| Abstract: | Two strains of Giardia(GD1 and GD2) isolated from feces of naturally infected dogs in Guangdong were genotyped by nested PCR of tpi gene.The genomic DNA of Giardia identified by the iodine staining was extracted and the tpi gene was amplified by using nested PCR assay.The BLAST and DNAStar softwares were used to analyze their homology and phylogeny.As demonstrated by tpi gene analysis,the homology of GD1 strain with L02120 was 100%,and they situated at the same branch of the phylogenic tree,while that of GD... |
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| Keywords: | dog Giardia lamblia tpi gene nested PCR |
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