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新城疫病毒HN蛋白单克隆抗体的制备
引用本文:于杨,崔佳莹,丁晴微,崔鹏飞,李印,邓国华,任涛,陈化兰. 新城疫病毒HN蛋白单克隆抗体的制备[J]. 中国畜牧兽医, 2011, 38(9): 94-97
作者姓名:于杨  崔佳莹  丁晴微  崔鹏飞  李印  邓国华  任涛  陈化兰
作者单位:1. 华南农业大学兽医学院,广东广州 510642;2. 中国农业科学院哈尔滨兽医研究所,农业部动物流感重点开放实验室/兽医生物技术国家重点实验室,黑龙江哈尔滨 150001
基金项目:国家自然科学基金(31072319)
摘    要:为制备新城疫病毒HN蛋白的单克隆抗体,本研究采用新城疫病毒CK/AH/100/2010作为免疫原,免疫6~8周龄BALB/c小鼠,3次免疫后取脾细胞与SP2/0融合,用血凝抑制(HI)方法检测筛选,具有HI活性的单抗有6株:PX1-PX6。6株单抗与AIV、EDS等其它具有血凝活性的病毒的交叉血凝抑制试验结果表明,6株单抗具有良好的特异性。间接免疫荧光试验结果表明,PX1、PX3可与新城疫病毒CK/GD/263/2010 HN抗原发生特异性反应,而其它单抗不反应。

关 键 词:新城疫病毒  单克隆抗体  HN基因  HI  
收稿时间:2011-01-17

Preparation Monoclonal Antibodies of HN Protein Using Newcastle Disease Virus
YU Yang,CUI Jia-ying,DING Qing-wei,CUI Peng-fei,LI Yin,DENG Guo-hua,REN Tao,CHEN Hua-lan. Preparation Monoclonal Antibodies of HN Protein Using Newcastle Disease Virus[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(9): 94-97
Authors:YU Yang  CUI Jia-ying  DING Qing-wei  CUI Peng-fei  LI Yin  DENG Guo-hua  REN Tao  CHEN Hua-lan
Affiliation:1. South China Agricultural University, Guangzhou 510642, China;2. Animal Influenza Laboratory of the Ministry of Agriculture/State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
Abstract:In order to develop monoclonal antibodies of HN protein of Newcastle disease virus,BALB/c mice were immunized with CK/AH/100/2010 virus,and spleens were used for fusion with SP2/0.Six McAb cell strains of PX1 to PX6 were determined by hemagglutinin inhibition(HI).The results of HI cross-reaction with AIV,EDS and other HA virus suggested that the six McAbs had excellent specificity with NDV.The McAbs PX1,PX3 were shown to react with the HN antigen of NDV CK/GD/263/2010 in indirect-immunofluorescence assay.
Keywords:Newcastle disease virus  monoclonal antibody  HN gene  HI  
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