| 一株新饲用乳杆菌的分离鉴定及益生性研究 |
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| 引用本文： | 孙晓雯,刘庆,闫轶洁,王安如,汪攀. 一株新饲用乳杆菌的分离鉴定及益生性研究[J]. 中国畜牧兽医, 2011, 38(9): 30-33 |
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| 作者姓名： | 孙晓雯 刘庆 闫轶洁 王安如 汪攀 |
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| 作者单位： | 1. 北京大北农科技集团股份有限公司饲用微生物工程国家重点实验室,北京 100193;2. 中国农业大学农业生物技术国家重点实验室,北京 100193 |
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| 摘 要： | 从猪粪便中分离得到一株新饲用乳杆菌,对其16S rDNA基因进行了测序,该段基因序列已提交GenBank,登录号为HQ641209。将该菌株16S rDNA基因序列进行在线BLAST比对并与其他乳杆菌属菌株的16S rDNA序列建立系统发育树,结果显示该菌16S rDNA序列与NCBI公布的约氏乳杆菌Lactobacillus johnsonii (AB295648)的同源性为99.7%,因此该菌鉴定为约氏乳杆菌(Lactobacillus johnsonii),将其命名为"Lactobacillus johnsonii Sun001"。经测试,该菌株对E.coli K88、K99菌株的生长有抑制作用,在pH为2.5的模拟胃液中处理3 h存活率为68.8%,在0.3%胆盐浓度的模拟肠液中处理3 h存活率为21.4%。
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| 关 键 词： | 约氏乳杆菌 益生性 分离鉴定 |
| 收稿时间： | 2010-12-14 |
Isolation and Identification and Probiotic Characteristics Study of a New Feeding Lactobacillus |
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| SUN Xiao-wen,LIU Qing,YAN Yi-jie,WANG An-ru,WANG Pan. Isolation and Identification and Probiotic Characteristics Study of a New Feeding Lactobacillus[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(9): 30-33 |
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| Authors: | SUN Xiao-wen LIU Qing YAN Yi-jie WANG An-ru WANG Pan |
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| Affiliation: | 1. State Key Laboratory of Direct-Fed Microbial Engineering, Da Bei Nong Group, Beijing 100193, China;2. State Key Laboratoriy of Agrobiotechnology, China Agricultural University, Beijing 100193, China |
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| Abstract: | A new Lactobacillus was isolated from a faecal sample of pig.The 16S rDNA gene was sequenced,and it was submitted to GenBank,the accession number was HQ641209.Online BLAST with other Lactobacillus and phylogenetic analysis based on the fragment of 16S rDNA reveal that its sequence homology was 97.9% comparing with Lactobacillus johnsonii strain(AB295648).The isolated strain was identified as Lactobacillus johnsonii and named Lactobacillus johnsonii Sun001.L.johnsonii had high level tolerance to the simulate... |
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| Keywords: | Lactobacillus johnsonii probiotic isolation and identificaton |
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