| 猪戊型肝炎病毒ORF2部分片段原核表达载体的构建 |
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| 引用本文： | 郝宝成,兰喜,胡永浩,柳纪省,梁剑平.猪戊型肝炎病毒ORF2部分片段原核表达载体的构建[J].中国畜牧兽医,2011,38(10):214-217. |
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| 作者姓名： | 郝宝成 兰喜 胡永浩 柳纪省 梁剑平 |
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| 作者单位： | 1. 中国农业科学院兰州畜牧与兽药研究所 农业部兽用药物创制重点实验室 甘肃省新兽药工程重点实验室,甘肃兰州 730050;2. 中国农业科学院兰州兽医研究所,甘肃兰州 730046;3. 甘肃农业大学动物医学院,甘肃兰州 730070 |
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| 基金项目： | 国家“十一五”科技支撑计划项目(2006BAD04A05-05); 中央级公益性科研院所基本科研业务费专项资金(BRF090402) |
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| 摘 要： | 利用Protean软件对猪戊型肝炎病毒(hepatitis E virus,HEV)swCH189株的ORF2进行潜在抗原位点分析,选取381aa－623aa段作为表达片段,运用PCR方法从ORF2全长质粒中扩增出目的片段cp239,经纯化、连接等,成功构建了表达载体pET32a(+)-cp239,为下一步进行基因原核表达及其生物学活性分析奠定了基础。
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| 关 键 词： | 猪 戊型肝炎病毒 ORF2 表达载体构建 |
| 收稿时间： | 2011-03-01 |
The Construction of Prokaryotic Expression Vector of Swine Hepatitis E Virus ORF2 Fragment |
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| HAO Bao-cheng,LAN Xi,HU Yong-hao,LIU Ji-xing,LIANG Jian-ping.The Construction of Prokaryotic Expression Vector of Swine Hepatitis E Virus ORF2 Fragment[J].China Animal Husbandry & Veterinary Medicine,2011,38(10):214-217. |
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| Authors: | HAO Bao-cheng LAN Xi HU Yong-hao LIU Ji-xing LIANG Jian-ping |
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| Institution: | 1. Key Laboratory of New Animal Drug Project,Gansu Province/Key Laboratory of Veterinary Drug Discovery,Agricultural Ministry/Lanzhou Institute of Husbandry and Pharmaceutical, Chinese Academy of Agricultural Sciences, Lanzhou 730050,China;2. Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences, Lanzhou 730046,China;3. College of Veterinary,Medical Gansu Agricultural University,Lanzhou 730070,China |
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| Abstract: | The test used the Protean software to analyze the potential antigenic sites of ORF2 of HEV swCH189 strain,and selected 381aa to 623aa segment as the expression of target,the cp239 fragment was amplified from the full-length ORF2 plasmid by PCR,after purification,connection,and so on,the prokaryotic expression vector pET32a(+)-cp239 was successfully constructed.This work laid the foundation for the next step of the prokaryotic expression and analysis of biological activity. |
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| Keywords: | swine hepatitis E virus ORF2 construction of prokaryotic expression vector |
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