DNA fingerprinting in soybean (Glycine max (L.) Merrill) with oligonucleotide probes for simple repetitive sequences |
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| Authors: | T. Yanagisawa M. Hayashi A. Hirai K. Harada |
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| Affiliation: | (1) Faculty of Agriculture, The University of Tokyo, 113 Tokyo, Japan;(2) Faculty of Agriculture, Meiji University, 214 Kawasaki, Japan;(3) Department of Molecular Biology, National Institute of Agrobiological Resources, 305 Tsukuba, Japan;(4) Present address: Department of Crop Breeding, National Agriculture Research Center, 305 Tsukuba, Japan;(5) Present address: Faculty of Horticulture, Chiba Univ., 271 Matsudo, Japan |
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| Abstract: | Summary Soybean DNA fingerprints were analyzed by digoxigenin-labeled oligonucleotide probes complementary to simple repetitive sequences. The clearest and most polymorphic patterns were obtained with (AAT)6 as a probe, with which all 47 soybean cultivars tested could be distinguished. However, DNA fingerprints of individuals within cultivars showed the same pattern. Using (CT)8, (GAA)5 or (AAGG)4 as probes, clear polymorphic patterns among cultivars and accessions in the subgenus Soja (Glycine max and Glycine soja) were not observed, while quite different patterns were found in accessions in the subgenus Glycine. The results suggest that G. max and G. soja are closer in their genome structure. DNA fingerprints of reciprocal crosses between cultivars and accessions in the subgenus Soja were similar, and contained bands of both parents. In an F2 population from these crosses, such bands segregated in a Mendelian fashion. |
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| Keywords: | Glycine max DNA fingerprinting, cultivar identification simple repetitive sequences oligonucleotide probes soybean |
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