| 草菇开伞相关基因反义表达载体构建 |
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| 引用本文： | 谢宝贵,罗联忠. 草菇开伞相关基因反义表达载体构建[J]. 食用菌学报, 2005, 12(2): 9-13 |
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| 作者姓名： | 谢宝贵 罗联忠 |
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| 作者单位： | 1. 福建农林大学菌物研究中心,福州,350002
2. 福建农林大学菌物研究中心,福州,350002;中国热带农业科学院热带作物品种资源研究所,海南省儋州市,571737 |
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| 基金项目： | 福建省自然科学基金重点项目"草菇开伞基因克隆及反义基因构建"(编号:B0120002)的部分内容 |
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| 摘 要： | 以质粒pCAMBIA1301和pBI121为基础构建草菇表达载体pCB。把已克隆的草菇开伞相关基因DNA片段正反向插入pCB,获得pCB—opn15和pCB—opn39,经测序证实,pCB—opn15为正向插入,pCB—opn39为反向插入。
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| 关 键 词： | 相关基因 表达载体构建 草菇 开伞 反义 pCB DNA片段 插入 反向 |
| 文章编号： | 1005-9873(2005)02-0009-05 |
Antisense Expression Vector Construction of Volvariella volvacea Fruitbody Opening Related Sequence |
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| XIE Bao-Gui,LUO Lian-zhong. Antisense Expression Vector Construction of Volvariella volvacea Fruitbody Opening Related Sequence[J]. Acta Edulis Fungi, 2005, 12(2): 9-13 |
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| Authors: | XIE Bao-Gui LUO Lian-zhong |
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| Affiliation: | XIE Bao-gui~1,LUO Lian-zhong~ |
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| Abstract: | An expression vector of pCB for Volvariella volvacea was constructed with plasmid pCAMBIA1301and pBI121. The DNA fragment of fruitbody opening related gene (opn) was inserted to plasmid pCB by two orientation. Two recombined plasmids of pCB-opn 15 and pCB-opn 39 were obtained,and the sequencing results showed that pCB-opn 15 was inserted in positive orientation and pCB-opn 39 was inserted in inversion. |
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| Keywords: | Volvariella volvacea Expression vector Fruitbody opening related gene Antisense gene |
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