| 白花蛇舌草组织培养物多糖的提取与含量测定 |
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| 引用本文： | 李国平,杨鹭生,黄群策. 白花蛇舌草组织培养物多糖的提取与含量测定[J]. 安徽农学通报, 2007, 13(3): 57-58. DOI: 10.3969/j.issn.1007-7731.2007.03.026 |
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| 作者姓名： | 李国平 杨鹭生 黄群策 |
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| 作者单位： | 郑州大学离子束生物工程省重点实验室,河南郑州,450052;莆田学院环境与生命科学系,福建莆田,351100;莆田学院环境与生命科学系,福建莆田,351100;郑州大学离子束生物工程省重点实验室,河南郑州,450052 |
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| 基金项目： | 福建省自然科学基金，福建省莆田市科研项目 |
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| 摘 要： | 采用分光光度法测定并比较白花蛇舌草野生植株及其组织培养物中的多糖含量.结果表明,白花蛇舌草野生植株中的多糖含量为3.517%、试管苗中的多糖含量为3.041%、愈伤组织中的多糖含量为4.825%.白花蛇舌草野生植株与试管苗的多糖含量相当,愈伤组织中的多糖含量较野生植株的高.为通过细胞大量培养技术提取白花蛇舌草免疫多糖提供科学依据.
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| 关 键 词： | 白花蛇舌草 多糖 分光光度法 组织培养 |
| 文章编号： | 1007-7731(2007)03-57-02 |
| 修稿时间： | 2006-12-18 |
Extraction and content determination of polysaccharide in tissue cultures of Hedyotis diffusa |
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| Li Guoping,Yang Lusheng,Huang Qunce. Extraction and content determination of polysaccharide in tissue cultures of Hedyotis diffusa[J]. Auhui Agricultural Science Bulletin, 2007, 13(3): 57-58. DOI: 10.3969/j.issn.1007-7731.2007.03.026 |
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| Authors: | Li Guoping Yang Lusheng Huang Qunce |
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| Abstract: | Object To determine and compare the content of polysaccharides in wild plants and tissue cultures of Hedyotis diffusa.Methods Spectrophotometry was used.Results The content of polysaccharides in wild plants from local district,plantlets regenerated on MS basic medium supplemented with BA 3mg/L and NAA 0.01mg/L and calli subcultured on MS basic medium was 3.517%,3.041% and 4.825% respectively.Conclusion The content of polysaccharides in calli was higher than those in wild plants and plantlets.These works presented scientific principles for the production of immune polysaccharides in Hedyotis diffusa by means of mass cell culture technique. |
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| Keywords: | Hedyotis diffusa polysaccharide spectrophotomephy tissue culture |
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