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| 三种梨果皮总RNA提取方法的比较研究 | |
| 引用本文： | 王西成,乔玉山,欧春青,王斐,马力,姜淑苓. 三种梨果皮总RNA提取方法的比较研究[J]. 甘肃农业大学学报, 2010, 45(2) |
| 作者姓名： | 王西成 乔玉山 欧春青 王斐 马力 姜淑苓 |
| 作者单位： | 1. 农业部果树种质资源利用重点开放实验室,辽宁,兴城,125100;南京农业大学园艺学院,江苏,南京,210095 2. 南京农业大学园艺学院,江苏,南京,210095 3. 农业部果树种质资源利用重点开放实验室,辽宁,兴城,125100;中国农业科学院果树研究所,辽宁,兴城,125100 4. 中国农业科学院果树研究所,辽宁,兴城,125100 |
| 基金项目： | 国家科技支撑项目(2008BAD92B07);;国家863课题(2006BAD13B06-1);;院长基金 |
| 摘 要： | 以西洋梨品种‘早红考密斯’、‘香红蜜’和白梨品种‘华酥’成熟果实为试材,采用Plantol总RNA提取试剂盒法、改良CTAB法和SDS法提取果皮中总RNA,并通过凝胶电泳、核酸蛋白分析仪和RT-PCR检测所提取总RNA样品的质量.结果表明,Plantol试剂盒法和改良CTAB法提取的RNA,经RT-PCR后得到的条带清晰,与目的片段大小一致,可以满足基因表达分析等试验的要求;而SDS法所提取的部分RNA,RT-PCR扩增不到目的片段,难以达到进一步试验的要求. |
| 关 键 词： | 梨 果皮 总RNA 提取 |
Extraction of total RNA from pericarp of pears with three methods |
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| WANG Xi-cheng,QIAO Yu-shan,OU Chun-qing,WANG Fei,MA Li,JIANG Shu-ling. Extraction of total RNA from pericarp of pears with three methods[J]. Journal of Gansu Agricultural University, 2010, 45(2) | |
| Authors: | WANG Xi-cheng QIAO Yu-shan OU Chun-qing WANG Fei MA Li JIANG Shu-ling |
| Affiliation: | 1.Key Laboratory of Fruit Germplasm Resources Utilization;Ministry of Agriculture;The People's Republic of China;Xingcheng 125100;China;2.College of Horticulture;Nanjing Agricultural University;Nanjing 210095;3.Institute of Pomology;Chinese Academy of Agricultural Sciences;China |
| Abstract: | The pericarp of pear fruit(cvs.'Early Red Cornice'Xianghongmi' and 'Huasu') was used as material for extracting total RNA with Plantol total RNA extraction kit,improved CTAB method and SDS method.The quality of the total RNA was tested by nuclear acid/protein analyzer,gel electrophoresis and RT-PCR.The results showed that the RNA got from Plantol total RNA extraction kit and improved CTAB method had a better quality,with a distinct bands and the size as the same with target fragments,and could satisfy the ... |
| Keywords: | pear pericarp total RNA extraction |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |