海水养殖凡纳滨对虾肌肉组织蛋白质组学研究

[目的]建立分析海水养殖凡纳滨对虾肌肉组织蛋白的双向电泳图谱及技术体系。[方法]采用裂解、离心等抽提方法提取凡纳滨对虾肌肉蛋白样品，利用bradford蛋白定量试剂盒作蛋白定量后，进行一向等电聚焦电泳和二向聚丙烯酰胺凝胶电泳。电泳后的凝胶采用考马斯亮蓝染色，利用ImageScannerⅢ扫描仪扫描凝胶，获得凝胶图像。[结果]经双向电泳图谱分析显示，所获得的凡纳滨对虾肌肉蛋白点分布于pH值4．0—7．0之间，大部分蛋白为酸性蛋白，其中有部分蛋白可能存在同分异构体，高pH值区蛋白分布相对较少。[结论]首次获得海水养殖凡纳滨对虾肌肉蛋白质的双向电泳图谱，初步建立了海水养殖凡纳滨对虾肌肉组织双向电泳技术体系．可为进一步探索海水养殖凡纳滨对虾的生理生化机制及比较蛋白质组学研究提供理论依据和技术保障。

Study on the Proteomics of Maricultured Litopenaeus vannamei Muscle Tissue

[Objective] The study aimed to establish two-dimensional electrophoresis maps and technologic platform on muscle tissue of maricultured Litopenaeus vannamei.[Method]Protein specimens were isolated using extraction methods,such aslysis and centrifugation,and they were analyzed with Isoelectric Focusing Electrophoresis and SDS-PAGE after treatment in bradford protein quantitative test kit.Then the gel is stained by Coomassie Brilliant Blue and gel image was obtained using Image Scanner III Scanner.[Results]An...