| 反义LeEIL2基因表达载体的构建及在番茄中的转化 |
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| 引用本文： | 薛印革,朱本忠,薛萍,田慧琴,罗云波. 反义LeEIL2基因表达载体的构建及在番茄中的转化[J]. 河南农业科学, 2003, 0(10): 47-50 |
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| 作者姓名： | 薛印革 朱本忠 薛萍 田慧琴 罗云波 |
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| 作者单位： | 中国农业大学食品学院,北京,100083 |
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| 摘 要： | 为了研究LeEIL2基因在番茄果实成熟过程中的作用 ,将全长的番茄LeEIL2基因以反义方向构建到植物表达载体 pBI1 2 1上 ,经过酶切鉴定正确后 ,将该载体转入农杆菌EHA1 0 5中 ,通过农杆菌介导法转化番茄 ,经组织培养和抗性筛选获得再生植株 ,PCR检测再生植株 ,初步鉴定为阳性植株 ,为获得转反义LeEIL2番茄果实打下基础。
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| 关 键 词： | EIN3/EIL2 番茄转化 载体构建 |
| 修稿时间： | 2003-06-11 |
Construction of Plant Antisense Gene Expression Vector pBI121- LeEIL2 and Transformation in Tomato |
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| XUE Yin-ge,ZHU Ben-zhong,XUE Ping,TIAN Hui-qin,LUO Yun-bo. Construction of Plant Antisense Gene Expression Vector pBI121- LeEIL2 and Transformation in Tomato[J]. Journal of Henan Agricultural Sciences, 2003, 0(10): 47-50 |
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| Authors: | XUE Yin-ge ZHU Ben-zhong XUE Ping TIAN Hui-qin LUO Yun-bo |
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| Abstract: | In order to investigate the role of LeEIL2 in tomato fruit ripening,the full-length LeEIL2 gene was inserted in reverse orientation into expression vector pBI121,and subsequently confirmed with restriction enzyme analysis. The antisense expression vector pBI121-LeEIL2 was mobilized into Agrobacterium tumefacious EHA105 by chemical-based DNA direct transformation. Transgenic tomato was developed using Agrobacterium-mediated transformation with kanamycin resistance selection. The presence of antisense LeEIL2 in transformants were confirmed by PCR and transgenic tomato fruit will be obtained later. |
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| Keywords: | EIN3/EIL2 Transformation of tomato Construction of vector |
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