PM 7/87 (2) Ditylenchus destructor and Ditylenchus dipsaci

Specific scope

This Standard describes a diagnostic protocol for Ditylenchus destructor and Ditylenchus dipsaci. 1 This Standard should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols. Terms used are those in the EPPO Pictorial Glossary of Morphological Terms in Nematology. 2

Specific approval and amendment

Approved in 2008‐09. This revision was prepared on the basis of the IPPC Diagnostic Protocol adopted in 2015 on D. dipsaci and D. destructor (Annex 8 to ISPM 27 Diagnostic protocols for regulated pests). The EPPO Diagnostic Protocol is consistent with the text of the IPPC Standard for morphological identification for this species. For comparison with other species the IPPC table includes Ditylenchus africanus whereas the EPPO table includes Ditylenchus convallariae due to the different distribution of the species. The molecular tests for which there is experience in the EPPO region are described in full in the appendices (some of these are additional tests to those in the IPPC protocol). Reference is given to the IPPC protocol for tests for which there is little experience in the EPPO region. DNA barcoding is also included. Revision approved in 2017‐04.