Test performance study of isothermal amplification tests for the detection of Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ |
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| Authors: | N Mehle P Kogovšek F Constable K De Jonghe M Loiseau F Veratti C Marzachi L Ferretti E Angelini L Filippin E Sousa E Andrade J Hodgetts M Dickinson M Dermastia |
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| Institution: | 1. National Institute of Biology (NIB), Ljubljana, (Slovenia);2. Department of Economic Development, Jobs, Transport and Resources, AgriBio, Bundoora, Vic., (Australia);3. Institute for Agricultural and Fisheries Research (ILVO), Merelbeke, (Belgium);4. Plant Health Laboratory (ANSES), Angers Cedex 01, (France);5. Istituto per la Protezione Sostenibile delle Piante (CNR), Torino, (Italy);6. Centro di Ricerca per la Patologia Vegetale (CREA‐PAV), Roma, (Italy);7. Centro di Ricerca per la Viticoltura (CREA‐VIT), Conegliano (TV), (Italy);8. Instituto Nacional de Investiga??o Agrária e Veterinária, I.P. (INIAV), Oeiras, (Portugal);9. Fera, National Agrifood, Innovation Campus, Sand Hutton, York, (United Kingdom);10. School of Biosciences, University of Nottingham, Loughborough, (United Kingdom) |
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| Abstract: | This test performance study (TPS) was carried out on DNA samples from grapevine, clematis, fungi and bacteria to compare and validate loop‐mediated isothermal amplification (LAMP) tests for detection of Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (Grapevine Bois noir phytoplasma). Two LAMP tests, for Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ (as developed by Kogov?ek and colleagues), with proven applicability for rapid laboratory or on‐site detection were included in this study. They were performed in 10 laboratories. In addition, the commercial Qualiplante/Hyris isothermal amplification test for Grapevine flavescence dorée phytoplasma was performed in three laboratories. The accuracy of the three tests was shown to be over 98%. Moreover, the high accuracy of these tests, which used different devices across different laboratories, confirmed their reproducibility. |
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