| 玉米自交系幼胚愈伤组织的诱导、分化及再生 |
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| 引用本文： | 闵丽,许崇香,安英辉,李伟忠,王红霞. 玉米自交系幼胚愈伤组织的诱导、分化及再生[J]. 杂粮作物, 2010, 30(3): 198-201 |
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| 作者姓名： | 闵丽 许崇香 安英辉 李伟忠 王红霞 |
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| 作者单位： | 黑龙江省农垦科研育种中心,黑龙江,哈尔滨,150036 |
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| 摘 要： | 取不同基因型的玉米自交系幼胚接种在诱导培养基上,结果表明：玉米幼胚愈伤组织的形成能力因其基因型的不同而呈显著差异,同时,愈伤组织的形成还与植物激素配比有关。其中,2,4-D是最重要的因素,适合的2,4-D浓度有利于愈伤组织的诱导。目前已经成功建立了部分玉米自交系幼胚的再生体系。
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| 关 键 词： | 玉米幼胚 胚性愈伤组织 再生植株 基因型 |
Callus Induction, Differentiation and Plant Regeneration of Immature Embryos of Maize Inbred Lines |
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| MIN Li,XU Chong-xiang,AN Ying-hui,LI Wei-zhong,WANG Hong-xia. Callus Induction, Differentiation and Plant Regeneration of Immature Embryos of Maize Inbred Lines[J]. Rain Fed Crops, 2010, 30(3): 198-201 |
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| Authors: | MIN Li XU Chong-xiang AN Ying-hui LI Wei-zhong WANG Hong-xia |
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| Affiliation: | (Reclamation Research Center of Crop Breeding of Heilongjiang Province,Harbin,Heilongjiang 150036,China) |
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| Abstract: | In this research,six varieties of maize（Zea mays L.） were chosen and the immature embryos were used to induce embryogenic callus.The factors affecting embryogenic callus regeneration were studied.The results were summarized that induction of embryogenic was inheritable.Different maize genotypes and the concentration of plant hormone showed different ability of embryogenic calli establishment and plant regeneration.On the whole,the concentration of 2,4-D was found to be most important factor for the induction of embryogenic calli.Maize regeneration system had established successfully. |
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| Keywords: | maize immature embryos embryogenic callus regeneration system genotype |
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