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| 生鲜乳中添加皮革水解蛋白的测定方法研究 | |
| 引用本文： | 刘学琴,徐栓朝,高建龙,刘维华. 生鲜乳中添加皮革水解蛋白的测定方法研究[J]. 乳业科学与技术, 2010, 33(4): 188-192 |
| 作者姓名： | 刘学琴 徐栓朝 高建龙 刘维华 |
| 作者单位： | 1. 宁夏水产研究所,宁夏银川,750001 2. 银川兴庆区农牧局,宁夏银川,750001 3. 宁夏动物食品质量安全检测中心,宁夏银川,750001 |
| 摘 要： | L-羟脯氨酸是皮革水解蛋白构成的特征氨基酸,因此鉴定L-羟脯氨酸就是鉴定皮革水解蛋白。建立UV法测定生鲜乳中皮革水解蛋白,L-羟脯氨酸浓度在0μg/mL～2.0μg/mL范围呈良好的线性关系,R=0.9998。L-羟脯氨酸平均回收率为100.2%,猪皮粉平均回收率为98.1%,皮革粉平均回收率为99.9%。该方法回收率高,检出限低,工作曲线稳定,重复性好。 |
| 关 键 词： | 生鲜乳 皮革水解蛋白 方法研究 |
Study on Determination Method of Protein Hydrolysis in Raw Milk Added Leather |
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| Liu Xueqin,Xu Shuanchao,Gao Jianlong,Liu Weihua. Study on Determination Method of Protein Hydrolysis in Raw Milk Added Leather[J]. JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY, 2010, 33(4): 188-192 | |
| Authors: | Liu Xueqin Xu Shuanchao Gao Jianlong Liu Weihua |
| Affiliation: | 1 Fisheries Research Institute of Ningxia, Yinchuan of Ningxia 750001, China; 2 Agriculture and Animai Husbandry of Xingqing ,Yinchuan of Ningxia 750001,China; 3 Ningxia Animal Food Quality and Safety Testing Center, Yinchuan of Ningxia 750001, China) |
| Abstract: | L-hydroxyproline is the characteristic of leather hydrolyzed protein amino acid, the identification is the identification of L-hydroxyproline leather hydrolyzed protein.UV method established in the leather raw and fresh milk hydrolyzed protein, L-hydroxyproline concentration in the range of 0 μg/mL～2.0 μg/mL good linear relationship, R=0.9998.L-hydroxyproline average recovery was 100.2 %, pig powder average recovery was 98.1 %, and leather powder average recovery was 99.9 %.This method was high in recovery, low in detection limit, stable-working curve and good-repeating. |
| Keywords: | raw milk protein hydrolysis method resea |
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