| PPV SC-1株VP2蛋白Cys-402、214的定点突变对VLPs的影响 |
| |
| 引用本文： | 朱玲,徐志文,郭万柱,胡秋炅,王印,王小玉.PPV SC-1株VP2蛋白Cys-402、214的定点突变对VLPs的影响[J].中国兽医学报,2010,30(3). |
| |
| 作者姓名： | 朱玲 徐志文 郭万柱 胡秋炅 王印 王小玉 |
| |
| 作者单位： | 四川农业大学,动物生物技术中心,四川,雅安,625014 |
| |
| 基金项目： | 国家自然科学基金资助项目(30500019); 四川省青年科技基金资助项目; 四川省教育厅重点实验室专项 |
| |
| 摘 要： | 以PPVVP2为基础,定点突变VP2蛋白中的Cys-402(A)、214(B),并构建突变体的真核表达载体,通过电镜技术和小鼠免疫试验探讨突变对VLPs的包装和免疫原性的影响。结果显示,在目标位点成功的引入突变,并构建了真核表达载体pPI-2-EGFP-VP2(A、B);电镜和小鼠免疫试验结果显示Cys-214对VLPs的组装和免疫原性的发挥是必需的,而Cys-402的突变并不干扰VLPs的形成和免疫原性的发挥,推测Cys-402及其附近可能存在利于外源基因插入的位点。本试验为以PPVVLPs为基础的多价疫苗和抗原转运系统提供了理论依据。
|
| 关 键 词： | 猪细小病毒 VP2 病毒样颗粒 定点突变 |
The site-directed mutation of Cys-402,214 of porcine parvovirus strain SC-1 and research on the effect of VLPs |
| |
| ZHU Ling,XU Zhi-wen,GUO Wan-zhu,HU Qiu-jiong,WANG Yin,WANG Xiao-yu.The site-directed mutation of Cys-402,214 of porcine parvovirus strain SC-1 and research on the effect of VLPs[J].Chinese Journal of Veterinary Science,2010,30(3). |
| |
| Authors: | ZHU Ling XU Zhi-wen GUO Wan-zhu HU Qiu-jiong WANG Yin WANG Xiao-yu |
| |
| Institution: | ZHU Ling,XU Zhi-wen,GUO Wan-zhu,HU Qiu-jiong,WANG Yin,WANG Xiao-yu (Animal Biological Technological Center,Sichuan Agricultural University,Ya\'an Sichuan,625014,China) |
| |
| Abstract: | Based on the porcine parvovirus(PPV) VP2,site-directed mutation was imported on Cys-402(A) and Cys-214(B),and then eukaryotic expression vectors of the mutants was constructed.Results:the mutations have been successfully imported and the eukaryotic expression vectors pPI-2-EGFP-VP2(A) and pPI-2-EGFP-VP2(B) were constructed;the result of electron microscope and BALB/c mice immuity test displayed that Cys-214 played an important role in VLPs' formation and immunogenicity;inversely,the mutation of Cys-402 was ... |
| |
| Keywords: | porcine parvovirus VP2 virus like-particles site-directed mutation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
