Identification of a telomere sequence type in three sponge species (Porifera) by fluorescence <Emphasis Type="Italic">in situ</Emphasis> hybridization analysis |
| |
Authors: | Mizuho Sakai Sei-Ichi Okumura Kanami Onuma Hiroaki Senbokuya Kunio Yamamori |
| |
Institution: | (1) School of Fisheries Sciences, Kitasato University, Sanriku, 022-0101 Ofunato Iwate, Japan |
| |
Abstract: | The telomere sequence type (TTAGGG)n is known to be distributed in various phyla in the Animalia and in Mastigophora (Protista). However, the telomere type of
Porifera (sponges), a phylum comprising the lowest multicellular animals, has not been reported. In this study, we examined
the three sponge species Leucetta chagosensis, Halichondria japonica, and Halichondria panicea for the presence of the telomere type (TTAGGG)n by fluorescence in situ hybridization (FISH). The oligonucleotide probe (TTAGGG)7 clearly displayed signals on the interphase nuclei of all three sponges. In contrast, the (TTAGG)7 probe, which has one base fewer than (TTAGGG)7, did not display the signals. These results suggest that the telomeres of the three sponges consist of (TTAGGG)n, which is identical to the sequence type found in many higher multicellular animals and in Mastigophora. Additionally, this
is the first study to reveal a telomere sequence type for Porifera. Moreover, these results suggest that Porifera are phylogenetically
related to Mastigophora, and supports the general theory that Porifera evolved from Mastigophora. Further, this study strongly
suggests that the origin of the (TTAGGG)n telomere sequence is to be found in a common ancestor of either the Bilateria and Porifera, or the Protista. |
| |
Keywords: | colonial theory fluorescence in situ hybridization invertebrate Mastigophora Porifera sponge telomere |
本文献已被 SpringerLink 等数据库收录! |
|