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| SSR标记用于贵州省喀斯特山区玉米自交系的遗传多样性分析 | |
| 引用本文： | 田彤,叶雨盛,曾桂萍,陈徇,李哲,戴保威,王晓琳. SSR标记用于贵州省喀斯特山区玉米自交系的遗传多样性分析[J]. 辽宁农业科学, 2011, 0(3): 32-35 |
| 作者姓名： | 田彤 叶雨盛 曾桂萍 陈徇 李哲 戴保威 王晓琳 |
| 作者单位： | 1. 岫岩县药山镇农业服务中心,辽宁岫岩,114306 2. 辽宁省农业科学院玉米研究所,辽宁沈阳,110161 3. 贵州大学农学院,贵州贵阳,550025 4. 辽宁省农业科学院生命科学中心,辽宁沈阳,110161 |
| 基金项目： | 科技部支撑计划(2006BAD01A03) |
| 摘 要： | 利用SSR标记对19个喀斯特山区常用玉米自交系进行遗传多样性分析,在此基础上又进行了杂种优势类划分。从90对引物中共筛选出46个能够稳定扩增的多态性引物,这46对引物共扩增出190条具有遗传多态性的条带,平均每个位点监测到的等位基因数为4.13个,变化范围为2～8个。每个位点的多态性信息量（PIC）变化于0.291～0.861之间,平均为0.623。供试材料的遗传距离变化范围为0.093～0.521,平均为0.342。UPGMA聚类分析结果表明,可将19个喀斯特山区常用玉米自交系划分为3个类群,分类结果与系谱来源基本一致。研究表明SSR标记可以进行玉米自交系遗传多样性分析。喀斯特山区地方玉米种质与温带玉米种质的遗传基础存在异质性,是温带玉米育种不可多得的异源种质。 |
| 关 键 词： | 玉米自交系 SSR标记 喀斯特山区 聚类分析 遗传多样性 |
Analysis on Genetic Diversity of Maize Inbred Lines in Karst Mountainous Area by SSR Markers |
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| TIAN Tong,YE Yu-sheng,ZENG Gui-ping,CHEN Xun,LI Zhe,DAI Bao-wei,WANG Xiao-lin. Analysis on Genetic Diversity of Maize Inbred Lines in Karst Mountainous Area by SSR Markers[J]. Liaoning Agricultural Sciences, 2011, 0(3): 32-35 | |
| Authors: | TIAN Tong YE Yu-sheng ZENG Gui-ping CHEN Xun LI Zhe DAI Bao-wei WANG Xiao-lin |
| Affiliation: | TIAN Tong1,YE Yu-sheng2,ZENG Gui-ping3,CHEN Xun4,LI Zhe2,DAI Bao-wei3,WANG Xiao-lin2(1.Yaoshan Agriculture Service Center of Xiuyan County Xiuyan,Liaoning 114306,2.Corn Research Institute of Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161,3.Agricultural College,Guizhou University,Guiyang,Guizhou 550025,4.College of Life Science,Liaoning Academy of Agricultural Sciences,Liaoning 110161) |
| Abstract: | The aim was to provide the basis for the utilization of the Karst Mountainous Area local maize germplasm.The technique of SSR markers was used to detected the genetic diversity of 19 maize inbred lines,which were widely used in karst mountainous area.46 SSR primers selected from 90 primers gave stable profiles am plified in sample of 19 maize inbred lines.A total of 190 polymorphic bands were detected by 46 primers selected.The average number of allele per SSR locus was 4.13 with a range from 2 to 8.The pol... |
| Keywords: | Maize inbred line SSR marker Karst mountainous area Cluster analysis Genetic diversity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |