甘蔗组织培养外植体的防褐化措施

为了抑制甘蔗外植体在培养过程中产生的褐化,保证其的正常生长分化,就甘蔗不同部位的茎尖及不同的抗氧化剂进行了研究.结果表明,甘蔗幼嫩的带芽茎段萌生的腋芽（5～8位芽）适宜做外植体;用0.75 g·L^-1的抗坏血酸对外植体进行预处理30 min,可以有效地防止褐化的产生,且不影响外植体的生长分化.

Control of Browning in Tissue Culture of Sugarcane (Saccharum officinarum L.)

Browning is a restraint for mass propatation of sugarcane by tissue culture. For successful tissue cul- ture of sugarcane it is very important to inhibit browning of sugarcane under tissue culture and make sure the tips of sugarcane stem grow and differentiate well. In this experiment axillary buds at different positions of stem sug- arcane were used as explants and pretreated with different antioxidants, ie. , ascorbic acid （VitC）, PVP, Na2S2O3 · 5H2O. The results showed that the axillary buds germinated from the young canes grew well and can be a good choice for explants, and that the pretreatment of the explants with VitC （0.75 g ·L^1 ） controlled browning of sugarcane efficiently and did not give impacts on the growth of the explants.