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红掌细菌性叶枯病病原菌的分离与鉴定
引用本文:梁丽雯,任希望,高志亮,符秀梅,林道哲,罗红丽,陈银华.红掌细菌性叶枯病病原菌的分离与鉴定[J].热带生物学报,2013,4(4):327-331.
作者姓名:梁丽雯  任希望  高志亮  符秀梅  林道哲  罗红丽  陈银华
作者单位:[1]海南大学海南省热带生物资源可持续利用重点实验室,海南海口570228 [2]海南大学农学院,海南海口570228
基金项目:国家自然基金(31060040,31260345);海南省重大科技专项(ZDZX2013023-02)
摘    要:采用组织分离法,从发病的红掌叶片上分离、纯化病原菌,对纯化的病原菌进行菌落形态、致病性、生化特性测定,并对细菌特异基因ABC transporter进行分离及序列分析.结果表明,分离得到的病原菌为地毯草黄单胞菌属细菌,为红掌的致病菌.

关 键 词:红掌  细菌性叶枯病  病原菌鉴定  地毯草黄单胞菌属(Xanthomonas  axonopodis)

Isolation and Characterization of Leaf Bacterial Blight Pathogen from Anthurium andraeanum
LIANG Liwen,REN Xiwang,GAO Zhiliang,FU Xiumei,LIN Daozhe,LUO Hongli,CHEN Yinhua.Isolation and Characterization of Leaf Bacterial Blight Pathogen from Anthurium andraeanum[J].Journal of Tropical Biology,2013,4(4):327-331.
Authors:LIANG Liwen  REN Xiwang  GAO Zhiliang  FU Xiumei  LIN Daozhe  LUO Hongli  CHEN Yinhua
Institution:1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Haikou 570225 , China; 2. College of Agronomy, Hainan University, Haikou 570228, China)
Abstract:Bacterial leaf blight is a destructive disease of Anthurium spp. This disease, caused by Xanthomonas, Pseudomonas or Erwinia, had a major impact on the production of Anthurium industry in China. Pathogens were isolated from the leaves of Anthurium andraenum infected with Bacterial leaf blight at the Anthurium nursery in Hainan, China. The pathogens were purified for morphological observation, biochemical determination of patho- genic characteristics, and specific bacterial ABC transporter gene analysis. The results showed that the isolated pathogens were Xanthomonas axonopodis.
Keywords:Anthurium andraeanum  leaf bacterial blight  pathogen identification  Xanthomonas axonopodis
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