唐菖蒲茎尖脱毒培养技术及其在生产上的应用

唐菖蒲种球微小茎尖在MS＋6-BA1．0～1．5mg·L-1＋NAA0．1—0．15mg·L-1的培养基上可诱导出丛生芽，每25d继代扩繁1次，增殖系数为3～4倍；将无根苗接种到1／2MS＋NAA0．3mg·L-1的培养基上，15～20d便可形成良好的根系；继续培养3—4个月，可形成试管小球茎。经检测，供试的5个品种的组培苗均未发现感染菜豆黄花叶病毒、黄瓜花叶病毒、烟草环斑病毒和蚕豆萎焉病毒，获得无病毒植株。将无毒试管小球茎作为原原种，在隔离网内进行栽培繁殖，可为大田生产提供无毒种球。

Tissue Culture of Gladiolus hybrids Hort with Shoot Tips as Explants

The shoot tips of the bulbs of Gladiolus hybrids were selected as explants for tissue culture. They were cultured on MS medium containing 1 -1.5 mg L-1 BA and 0.1 -0. 15 mg L- NAA and induced to produce clustered buds. The clustered shoots were proliferated 3 to 4 times every 25 days after the clustered buds were subcuhured. Rootless plantlets formed a strong root system on the 1/2 MS culture medium supplemented with 0.3 mg L-1 NAA in 15 to 20 days, and then produced in vitro bulbs after 3 to 4 months of subculture. Virus detection showed that the in vitro bulbs of 5 varieties of G. hybrids were free of bean yellow mosaic virus （BYMV）, cucumber mosaic virus （CMV）, tobacco ring spot virus （TRSV） and broad bean wilt virus （BB- WV）. These virus- free in vitro bulbs （initial bulbs） were cultivated in the net house for propagation of G. hy- brids. The virus-free bulbs propagated from the bulbs in the net house can be used for commercial cultivation in the field.