Antioxidant capacity manipulation in transgenic potato tuber by changes in phenolic compounds content |
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Authors: | Lukaszewicz Marcin Matysiak-Kata Iwona Skala Jacek Fecka Izabela Cisowski Wojciech Szopa Jan |
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Affiliation: | Institute of Genetics and Microbiology and Institute of Biochemistry and Molecular Biology, Wroclaw University, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland. |
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Abstract: | The main goal of this study was to generate potato tubers with increased levels of flavonoids and thus modified antioxidant capacities. To accomplish this, the vector carrying multigene construct was prepared and several transgenic plants were generated, all overexpressing key biosynthesis pathway enzymes. The single-gene overexpression or simultaneous expression of genes encoding chalcone synthase (CHS), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR) resulted in a significant increase of measured phenolic acids and anthocyanins. The increase in phenolic compounds synthesis is accompanied by decreases in starch and glucose levels in transgenic plants. The flavonoids-enriched plants showed improved antioxidant capacity; however, there is a complex relationship between antioxidant capacity and flavonoids content, suggesting the great participation of other compounds in the antioxidant potential of the plants. These other compounds are not yet recognized. |
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