影响水稻纹枯病菌营养体亲和性分化的因子

 为了探明影响水稻纹枯病菌营养体亲和性分化的因子，将水稻纹枯病菌菌株cx-2在不同水稻品种继代接种，并在含不同杀菌剂、具不同pH及不同温度条件的PDA平板上继代培养，用对峙法测定继代菌株与原始接种菌株的营养体亲和性。此外，对营养体亲和性分化菌株与原始菌株的AFLP指纹图谱进行比较。结果显示，在供试的30个水稻品种上连续接种4次后，从9个水稻品种中分离出与原始菌株营养体不亲和的菌株。菌株在不同pH值的PDA平板继代培养4次后，在pH偏碱性端(pH 10、pH 11)开始出现营养体亲和性分化的菌株。在不同农药和温度条件下继代培养10次的菌株中没有分离到营养体亲和性分化的菌株。营养体亲和性分化菌株与原始菌株的AFLP指纹图谱没有差异。

The impact factors on differentiation of somatic compatibility of Rhizoctonia solani AG-1-IA

To clarify the factors influencing differentiation of somatic compatibility in Rhizoctonia solani AG-1-IA causing rice sheath blight, the pathogen strain cx-2 was consecutively inoculated into different rice varieties and subcultured on PDA plates with different fungicides/pHs or at different temperatures. The comparison of somatic compatibility was made between the recovered/subcultured isolates and their original strain (cx-2) based on barrage zone formation on the face-to-face cultures. Meanwhile, amplified fragment length polymorphism (AFLP) was used to determine genetic difference between the recovered/subcultured isolates with alterations in somatic compatibility and their original strain (cx-2). The results showed that the isolates with alterations in somatic compatibility were recovered from 9 out of 30 rice varieties tested after four consecutive inoculations. The subcultured isolates with alterations in somatic compatibility were also detected after four consecutive transfers on the PDA plates with alkaline pH levels 10 and 11. However, no subcultured isolates with alterations in somatic compatibility were found even after ten consecutive transfers on the PDA plates containing different fungicides or at different temperatures. The isolates with alterations in somatic compatibility showed the same AFLP fingerprint patterns with their original strain (cx-2).