| 谷氨酸棒杆菌麦芽寡糖基海藻糖合成酶基因的克隆与表达 |
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| 引用本文： | 张文德,乔宇,丁宏标.谷氨酸棒杆菌麦芽寡糖基海藻糖合成酶基因的克隆与表达[J].中国农业科技导报,2009,11(1):68-72. |
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| 作者姓名： | 张文德 乔宇 丁宏标 |
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| 作者单位： | (中国农业科学院饲料研究所, 北京100081) |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国际科技合作重点项目 |
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| 摘 要： | 采用PCR方法从谷氨酸棒杆菌（Corynebacterium glutamicum）基因组中分离得到2.4 kb的麦芽寡糖基
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| 关 键 词： | 麦芽寡糖基海藻糖合成酶 麦芽糊精 谷氨酸棒杆菌 重组表达 |
| 收稿时间： | 2008-11-14 |
| 修稿时间： | 2008-12-26 |
Cloning and Expression of Gene Encoding Maltoologosyl Trehalose
Synthase from Corynebacterium glutamicum |
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| ZHANG Wen-de,QIAO Yu,DING Hong-biao.Cloning and Expression of Gene Encoding Maltoologosyl Trehalose
Synthase from Corynebacterium glutamicum[J].Journal of Agricultural Science and Technology,2009,11(1):68-72. |
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| Authors: | ZHANG Wen-de QIAO Yu DING Hong-biao |
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| Institution: | ( Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081,China) |
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| Abstract: | A 2.4 kb DNA fragment encoding maltooligosyl trehalose synthase was cloned from
Corynebacterium glutamicum by PCR. It was inserted into prokaryotic expression vector
pET-30a and then was introduced into the host Escherichia coli BL21 (DE3) pLysS. A high
yield of the recombinant enzyme at a molecular weight of 90 kDa was obtained by IPTG
induction. The soluble recombinant enzyme accounted for 45.3% of the total cell proteins
in supernatant. This recombinant enzyme was capable of decreasing the DE value of dextrin
and producing a maltooligosyl trehalose mixture. |
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| Keywords: | maltoologosyl trehalose synthase malt dextrin Corynebacterium glutamicum recombinant expression |
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