Differences of EST-SSR and genomic-SSR markers in assessing genetic diversity in poplar |
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| Authors: | Yue-peng Song Xi-bing Jiang Man Zhang Ze-liang Wang Wen-hao Bo Xin-min An De-qiang Zhang Zhi-yi Zhang |
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| Institution: | 1 National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administra- tion, Beijing Forestry University, Beijing 100083, P. R. China 2 College of Horticulture, Northwest Agriculture and Forestry University, Yangling 712100, P. R. China |
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| Abstract: | We analyzed the genetic differences of 16 poplar clones between genomic-SSR and EST-SSR markers. The statistical results show
that the average number of alleles detected by genomic-SSR was 4.1, Shannon’s index 1.0646, observed heterozygosity 0.4427
and expected heterozygosity 0.5523, while for the EST-SSR, the average number of alleles was 2.8, Shannon’s index 0.6985,
observed heterozygosity 0.2330 and expected heterozygosity 0.4684. Cluster analysis indicated that the EST-SSR capacity of
genotypic identification was more precise than that of genomic-SSR. These results reveal that EST-SSR and genomic-SSR have
statistically significant genetic differences in polymorphism detection and genotypic identification. These differences could
provide a theoretical basis for the rational use of SSR markers in species diversity and other related research. |
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| Keywords: | poplar genomic-SSR EST-SSR genetic differences |
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