Isolation and characterization of eight microsatellite loci in the Otton frog, Babina subaspera

The Otton frog, Babina subaspera, is an endangered forest frog species with unusual morphological and ecological features compared with other frogs. Eight microsatellite markers were isolated from B. subaspera and characterized with 24 individuals from Amami Islands, Kagoshima prefecture, Japan. The number of alleles observed for each locus ranged from 9 to 19 (average 13.9). The observed heterozygosity (H _O) and the expected heterozygosity (H _E) ranged from 0.71 to 0.92 and 0.83 to 0.92, respectively. These microsatellites will be useful for future genetic studies of B. subaspera, which will be essential in the conservation of this species.