| BADH基因转化水稻的方法比较 |
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| 引用本文： | 卢德赵,祝水金,钱前,王慧中,颜美仙,黄大年. BADH基因转化水稻的方法比较[J]. 中国水稻科学, 2003, 17(4): 323-327 |
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| 作者姓名： | 卢德赵 祝水金 钱前 王慧中 颜美仙 黄大年 |
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| 作者单位： | 1.中国水稻研究所 农业部水稻生物学重点实验室， 浙江 杭州 310006； 2浙江大学 农业与生物技术学院 农学系， 浙江 杭州 310029;3.浙江中医学院 生命科学系， 浙江 310053； 4 杭州师范学院 生物科学系， 浙江 杭州 310036) |
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| 基金项目： | 国家转基因专项资助项目 (JY 0 3 B 0 8)，浙江省自然科学基金资助项目，中国水稻科学基金资助项目 |
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| 摘 要： | 应用基因枪法与农杆菌介导法将来源于山菠菜的BADH（betaine aldehyde dehydrogenase）基因导入水稻。从获得转基因植株数量、转化率、转化植株PCR阳性率、外源基因拷贝数及其表达活性、T0代植株的结实率对两种转化方法进行了比较。结果表明，用农杆菌介导法转化BADH基因的转化效率和PCR阳性率比基因枪法更高，且用农杆菌介导法得到的转基因植株T0的结实率等农艺性状也优于基因枪法。
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| 关 键 词： | 水稻 甜菜碱醛脱氢酶基因 转化 基因枪法 农杆菌介导法 方法 |
| 文章编号： | 1001-7216(2003)04-0323-05 |
| 收稿时间： | 1900-01-01; |
Research on the Methods for Transforming BADH Gene into Rice |
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| LU De-zhao ,,,ZHU Shui-jin ,QIAN Qian ,WANG Hui-zhong ,YAN Mei-xian ,HU ANG Da-nian. Research on the Methods for Transforming BADH Gene into Rice[J]. Chinese Journal of Rice Science, 2003, 17(4): 323-327 |
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| Authors: | LU De-zhao ZHU Shui-jin QIAN Qian WANG Hui-zhong YAN Mei-xian HU ANG Da-nian |
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| Affiliation: | （;1. Key Laboratory for Rice Biology; Ministry of Agriculture; P.R. China; China National Rice Research Institute; Hangzhou 310006; China;2. Department of Agronomy; College of Agriculture and Biotechnology; Zhejiang University; Hangzhou 310029; China;3.Department of Life Science; Zhejiang College of Traditional Chinese Medicine; Hangzhou 310053;4.Department of Biology, Hangzhou Normal College, Hangzhou 310036, China) |
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| Abstract: | The transformation effects of BADH gene (encod ing betaine aldehyde dehydrogenase) into rice by particle bombarment and Agr obacterium tumefaciens mediated were compared in transgenic plant number, t ransformation efficiency, rate of PCR positive plantlets, foreign gene copies and their expression activity, and seed setting rate of T 0 generation. Resu lts showed that transformation efficiency and rate of PCR positive plantlets by Agrobacterium tumefaciens mediated were higher than those by particle bombardment method. Moreover, the performance in seed setting rate of T 0 ge neration and some other agronomic traits of transgenic rice obtained from A grobacterium tumefaciens mediated gene transfer system were better compared w ith particle bombarment methodology. |
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| Keywords: | rice betaine aldehyde dehydrogenase gene transfor mation particle bombarment Agrobacterium tumefaciens mediated methodology |
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