紫花苜蓿耐盐分子标记的初步鉴定

 以耐盐苜蓿与敏盐苜蓿相互杂交的F2群体为试验材料，利用改良的BSA 法和RAPD技术筛选出一个与苜蓿耐盐基因相连锁的分子标记。将该标记的PCR产物进行了DNA序列测序，在GenBank中对该片段与其它相关基因进行比对分析表明，苜蓿耐盐标记的核苷酸序列与截形苜蓿（Medicago truncatula）的mth2-6e18基因的一个片段（347 bp）的序列有93%的同源性。mth2-6e18基因是植物干旱、盐诱导半胱氨酸蛋白酶（CysPr1）基因的标记基因，推测该片段与植物干旱、盐诱导的CysPr1基因具有较大的相关性。

Identification of Molecular Marker to Salt Tolerance Gene in Alfalfa

The study has established the F2 offspring oblained by crossing salt-tolerant alfalfa with salt-sensitive alfalfa, and the salt-tolerant F2 offspring seedlings was evaluated in a pot culture. With the F2 offspring, the study has obtained molecular marker linked with salt-tolerant genes of alfalfa by using the improved BSA and RAPD marker. The RAPD PCR products were recovered and purified using kit, then were sequenced. The DNA sequence was aligned in GenBank, the result of alignment showed that the DNA sequence was homological with one fragment (347 bp) of the mth2-6e18 of Medicago truncatula, and the similarity were 93%. mth2-6e18 is a molecular marker of cysteine proteases that plant salt inducible gene translated. It is extrapolated that this molecular marker has a significant correlation with cysteine proteases gene.