| 黔北麻羊遗传结构的RAPD分析 |
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| 引用本文： | 陈祥,张芸,廖正录,张勇,李国红,李诗举,王学伟,简承松,魏泓.黔北麻羊遗传结构的RAPD分析[J].西南农业大学学报,2007,29(2):53-56. |
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| 作者姓名： | 陈祥 张芸 廖正录 张勇 李国红 李诗举 王学伟 简承松 魏泓 |
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| 作者单位： | [1]贵州大学动物科学学院,贵阳550025 [2]贵州省农业厅畜禽品种改良站,贵阳550001 [3]贵州师范大学生物系,贵阳550001 [4]第三军医大学实验动物中心,重庆400038 |
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| 基金项目： | 国家自然科学基金资助项目（30070120）;贵州省科学技术基金[黔科合J字（2005）2031号];[黔基合计字（2002）305号]. |
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| 摘 要： | 用27条多态性引物对15份黔北麻羊个体基因组进行RAPD分析．结果共扩增出253条带，其中多态带为141条，多态频率在30．00%～75．00%之间，平均多态频率为55．73%．单个引物获得的扩增带数在2～14条之间，平均每条引物扩增出9．37条带．扩增带分子量范围在210～2700bp．Nei氏公式计算品种内个体间遗传相似性指数在0．7290～0．9377之间，平均值为0．8634．遗传距离指数在0．0623～0．2710之间，平均值为0．1367．结果表明黔北麻羊具有较丰富的遗传结构．
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| 关 键 词： | 黔北麻羊 遗传多样性 随机扩增多态脱氧核糖核酸 |
| 文章编号： | 1000-2642（2007）02-0053-04 |
| 修稿时间： | 2006-07-02 |
Rapd Analysis of the Genetic Structure of Qianbei-Pockmarked Goat Population |
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| CHEN Xiang, ZHANG Yun, LIAO Zheng-lu, ZHANG Yong, LI Guo-hong, LI Shi-ju, WANG Xue-wei, JIAN Cheng-song, WEI Hong.Rapd Analysis of the Genetic Structure of Qianbei-Pockmarked Goat Population[J].Journal of Southwest Agricultural University,2007,29(2):53-56. |
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| Authors: | CHEN Xiang ZHANG Yun LIAO Zheng-lu ZHANG Yong LI Guo-hong LI Shi-ju WANG Xue-wei JIAN Cheng-song WEI Hong |
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| Institution: | 1. College of Animal science, Guizhou University, Guiyang 550025, China; 2. Guizhou Provincial Livestock Bureau, Guiyang 550001, China; 3. Dept. of Biology, Guizhou Normal University, Guiyang 550001, China; 4. Laboratory Animal Center, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | Twenty-seven polymorphic primers were used to amplify the genome DNA of 15 Qianbei-Pockmarked goats, and 253 bands were detected, of which 141 were polymorphic with a polymorphism frequency range of 30.00%~75.00%, averaging 55.73%. Each primer amplified 2~14 bands, averaging 9.37. The fragment length of the amplified products ranged between 210 and 2 700 bp. Calculated by Nei formulae, the genetic similarity index ranged from 0.729 0 to 0.937 7, averaging 0.863 4, and the genetic distance index was between 0.062 3 and 0. 271 0, averaging 0. 136 7. These results indicate that Qianbei-Pockmarked goats have high inter-individual genetic variation and abundant genetic structures. |
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| Keywords: | Qianbei-Pockmarked goat random amplified-polymorphic DNA genetic variation |
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