| 利用分子标记定位普通菜豆抗炭疽病基因 |
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| 引用本文： | 陈明丽,王兰芬,王晓鸣,张晓艳,王述民. 利用分子标记定位普通菜豆抗炭疽病基因[J]. 作物学报, 2011, 37(12): 2130-2135. DOI: 10.3724/SP.J.1006.2011.02130 |
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| 作者姓名： | 陈明丽 王兰芬 王晓鸣 张晓艳 王述民 |
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| 作者单位： | 1江苏沿江地区农业科学研究所, 江苏如皋 226541; 2扬州大学作物遗传生理省级重点实验室, 江苏扬州 225009 |
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| 基金项目： | 国家科技支撑计划项目，农业部作物种质资源保护与利用专项资助 |
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| 摘 要： | 为了定位中国普通菜豆的抗炭疽病基因, 选取抗炭疽病地方品种红芸豆(国家库编号F2322)与高感菜豆品种京豆(国家库编号F0777)配制杂交组合, 构建F2抗感分离群体和F2:3家系, 用菜豆炭疽菌81号生理小种鉴定抗病性并分析遗传性。结果表明, 红芸豆对菜豆炭疽菌81号小种的抗性是由一显性单基因控制的, 暂将该基因命名为Co-F2322。用分离群体分组分析法(BSA)和SSR、CAPs分子标记技术, 将该基因定位在B1连锁群上, 利用软件Mapmaker 3.0和Mapchart 3.0计算标记与目的基因间的遗传距离, 检测到3个SSR标记BMc32、C871、Pvm98和2个CAPs标记g1224、g683与抗炭疽病基因连锁, 遗传距离分别为26.06、3.58、13.56、3.81和12.75 cM。
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| 关 键 词： | 玉米自交系 粗缩病 抗性鉴定 遗传多样性 |
| 收稿时间： | 2011-04-19 |
Mapping of Gene Conferring Resistance to Anthracnose in Common Bean (Phaseolus vulgaris L.) by Molecular Markers |
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| CHEN Ming-Li,WANG Lan-Fen,WANG Xiao-Ming,ZHANG Xiao-Yan,WANG Shu-Min. Mapping of Gene Conferring Resistance to Anthracnose in Common Bean (Phaseolus vulgaris L.) by Molecular Markers[J]. Acta Agronomica Sinica, 2011, 37(12): 2130-2135. DOI: 10.3724/SP.J.1006.2011.02130 |
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| Authors: | CHEN Ming-Li WANG Lan-Fen WANG Xiao-Ming ZHANG Xiao-Yan WANG Shu-Min |
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| Affiliation: | 1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;2.Qingdao Academy of Agricultural Sciences, Qingdao 266100, China |
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| Abstract: | To locate gene conferring resistance to anthracnose caused by pathogenic fungus(Colletotrichum lindemuthianum) from Chinese common bean,we established F2 plants and F3 families derived from a cross between red common bean cultivar F2322(resistant to race 81) and Jing common bean cultivar F0777(susceptible to race 81),identified resistance,and did genetic analysis.The results showed that red bean carried a single dominant gene for resistance to anthracnose,designated as Co-F2322 tentatively.Molecular genetic... |
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| Keywords: | Common bean Anthracnose Bulked segregant analysis(BSA) Anthracnose resistant gene Molecular marker |
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