大豆逆境诱导基因GmPRP的克隆与表达

通过对大豆吉林32未成熟胚表达谱的分析，利用RT-PCR技术从大豆中克隆了一个新的脯氨酸富集蛋白基因，命名为GmPRP。GmPRP的开放阅读框长396 bp, 其分子量13.79 kD，具有131个氨基酸残基，等电点8.96，其DNA序列无内含子。GmPRP蛋白序列N端含有一段信号肽，中间为脯氨酸富集区，C端为半胱氨酸富集区。该蛋白与四季豆和木豆的PRP同源性最高, 具有较近的亲缘关系。GmPRP的683 bp启动子序列含有10种与逆境相关的顺式作用元件，分别为ABRE-like、G-box、W-box、GT-1、MYB、MYC、BIHD10s、DPBF、SEBF和WRKY。实时荧光定量PCR分析表明, 该基因表达量在大豆的根和叶中最高，在茎和胚中其次，在花中最低，且受干旱、高盐、低温、机械伤害及SA(水杨酸)、ETH(乙烯)、ABA(脱落酸)、MeJA (茉莉酸甲酯)的诱导上调表达。

Cloning and Expression of a Stress-induced GmPRP Gene in Soybean (Glycine max)

Plant proline-rich proteins(PRPs) are putative cell wall proteins,which are usually associated with different abiotic and biotic stress conditions.A soybean mRNA sequence encoding a proline-rich protein(PRP) was cloned and designated as GmPRP from Jilin 32 immature embryo gene expression profiles using RT-PCR.The GmPRP consisted of an ORF with a length of 396 bp,and encoded 131 amino acids(13.79 kD) with an isoelectric point of 8.96.There was no intron in the DNA sequence of GmPRP.Except a repetitive prolin...